摘要： 信号标签诱变技术是以整个基因组为基础的研究病原体致病机制，可在体内对毒力基因进行高通量筛选的一种新方法。近几年应用该技术已对十多种病原微生物进行了筛选。这些筛选中除了找到已知的毒力基因外，还都鉴定到了未知的毒力因子。本文就该技术的原理、优缺点、应用的必要条件、技术的改进及应用该技术鉴定到的毒力基因等作一综述。

A Novel Approach to Study Pathogenesis of Pathogens in vivo——Signature-tagged Mutagenesis
YAO Xiao1,2,HUANG Liu-yu1,YANG Bo-lun2,SU Guo-fu1
1.Beijing Institute of Biotechnoloy,Beijing 100071,China;
2.College of Environmental and Chemical Engineering,Xi'an Jiaotong University,Xi'an 710049,China
Abstract:Signature-tagged mutagenesis (STM) is a novel approach to study pathogenesis of pathogens and to screen virulence genes with high throughput in vivo,which is based on whole genome of pathogen in question.In resent years,more than ten species of microbial pathogens have been screened with this technology.There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens.This article reviews the principle,advantages and current limitations,the requirements,modifications of STM,and to date virulence genes identified by this technology.
Key words：signature-tagged mutagenesis;virulence genes;pathogens;in vivo

关键词: 信号标签诱变, 毒力基因, 病原体, 体内