双链探针同步荧光技术快速筛查C282Y点突变

遗传 ›› 2003, Vol. 25 ›› Issue (1): 9-13.

双链探针同步荧光技术快速筛查C282Y点突变

张永有;李庆阁;栾国彦;梁基选

厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室，厦门 361005

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-02-10 发布日期:2003-02-10

Screening C282Y Mutation with Double-Stranded Probes Using Synchronous Fluorometry

ZHANG Yong-You;LI Qing-Ge;LUAN Guo-Yan;LIANG Ji-Xuan

The Key Laboratory of Cell Biology and Tumor Cell Engineering of The Ministry of Education,School of Life Sciences,Xiamen University,Xiamen,Fujian 361005,China

Received:1900-01-01 Revised:1900-01-01 Online:2003-02-10 Published:2003-02-10

摘要/Abstract

摘要： 以荧光染料Fam和Joe分别标记野生型和突变型双链探针作为均相检测探针，以构建的DNA模板作为研究模型，采用固定波长差同步荧光分析法对PCR反应产物进行终点检测。通过对HFE基因C282Y点突变的检测，并以限制性内切核酸酶Rsa I证实，该方法是一种廉价、快速、可靠的筛查遗传性血色病基因C282Y突变的方法，该法可扩展到各种基因的突变检测。

关键词: 基因突变筛查, 同步荧光, 双链探针, 遗传性血色病

Abstract: We described in the paper a new high-throughput screening method for Cys282Tyr mutation in hereditary haemochromatosis with double-stranded probe using synchronous fluorometry.The probe for wild type was labeled with Fam,the probe for mutant type was labeled with Joe.After PCR,reaction tubes were transferred to a spectrofluorometer,where synchronous spectra were scanned in a constant-wavelength mode.The genotype could be obtained through the appearance of the fluorescence peaks corresponding to each probe.The results were totally in agreement with restriction endonuclease analysis.Considering the simplicity,low cost and specificity,this approach could be generally applied to detect varieties of gene mutations.

张永有，李庆阁，栾国彦，梁基选. 双链探针同步荧光技术快速筛查C282Y点突变[J]. 遗传, 2003, 25(1): 9-13.

ZHANG Yong-You, LI Qing-Ge, LUAN Guo-Yan, LIANG Ji-Xuan. Screening C282Y Mutation with Double-Stranded Probes Using Synchronous Fluorometry[J]. HEREDITAS, 2003, 25(1): 9-13.