遗传 ›› 2003, Vol. 25 ›› Issue (6): 703-704.

• 论文 • 上一篇    下一篇

一种简单高效的食用真菌总RNA提取方法

周凯松;薛久刚;常宁;陈畅;张晗星;张长铠 ZHOU Kai-Song;XUE Jiu-Gang;CHANG Ning;CHEN Chang;ZHANG Han-Xing;ZHANG Chang-Kai   

  1. 山东大学生命科学学院微生物技术国家重点实验室,济南 250100 State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100,China
  • 收稿日期:1900-01-01 出版日期:2003-12-10 发布日期:2003-12-10

A Simple and Effective Method for Extracting Total RNA from Domestic Fungus

  • Received:1900-01-01 Online:2003-12-10 Published:2003-12-10

摘要: 以金针菇为材料,建立了一种适合于富含RNase、多酚、多糖和糖蛋白的食用真菌RNA的提取方法,此方法在高浓度变性剂存在的条件下2次用苯酚-氯仿-异戊醇进行抽提去除DNA、蛋白质,并用异丙醇和乙酸钠选择性沉淀RNA、去除多糖,得到完整、均一的RNA样品。
Abstract:With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols,polymeric carbohydrates and proteoglycans..Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol,sodium acetate were used to precipitate RNA selectively.Pure and intact RNA can be effectively prepared by this method.

关键词: 食用菌, RNA提取, RNA extracting, domestic fungus, 金针菇
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