遗传 ›› 2004, Vol. 26 ›› Issue (1): 55-58.

• 论文 • 上一篇    下一篇

蒙古山羊和哈萨克山羊GOLA-DRB3基因的HaeⅢ酶切多态性分析

孙东晓1;张沅1;李宁2 SUN Dong-Xiao1;ZHANG Yuan1;LI Ning2   

  1. 1.中国农业大学动物科技学院,北京 100094; 2.中国农业大学农业生物技术国家重点实验室,北京 100094 1.College of Animal Science And Technology,China Agricultural University,Beijing 100094,China; 2.State Key Laboratory For Agro-Biotechnology,China Agricultral University,Beijing 100094,China
  • 收稿日期:1900-01-01 出版日期:2004-02-10 发布日期:2004-02-10

Polymorphism Analysis of the GOLA-DRB3 Gene Digested with HaeⅢ in Mongolian Goat and Kazakh Goat

  • Received:1900-01-01 Online:2004-02-10 Published:2004-02-10

摘要:
采用限制性内切核酸酶HaeⅢ对蒙古山羊和哈萨克山羊GOLA-DRB3基因外显子2的285bp扩增产物进行了PCR-RFLP多态性分析,共检测到17种基因型,由A、B、C、D、E、F和H等7个复等位基因控制;通过酶切图谱分析发现蒙古山羊和哈萨克山羊的GOLA-DRB3基因外显子2的154、168和220位碱基表现出多态性。并对基因型频率和等位基因频率进行了统计分析,结果表明,GOLA-DRB3基因的部分基因型频率和等位基因频率在两个群体之间差异显著(P<0.10或P<0.05)或极显著(P<0.01); χ2适合性检验结果表明,蒙古山羊和哈萨克山羊的GOLA-DRB3基因外显子2的HaeⅢ酶切位点均未达到Hardy-Weinberg平衡状态(P<0.01)。
Abstract:The exon2 of GOLA-DRB3 gene was amplified and a uniform fragment of 285bp was obtained in Mongolian Goat and Kazakh Goat.The 285bp PCR product was digested with restriction endomuclease HaeⅢ and genetic polymorphism was investigated by PCR-RFLP.Seventeen kinds of genotypes were found in two populations,which were controlled by seven alleles.There are significant differences in some genotypic frequencies and gene frequencies between the two populations(P<0.10,P<0.05,P<0.01);The results of χ2 test showed that genotypes of GOLA-DRB3 gene in two populations did not fit with Hardy-Weinberg equilibrium(P<0.01).

关键词: Mongolian goat, PCR-RFLP
Key words,
GOLA-DRB3, 哈萨克山羊, 蒙古山羊