蛋白质剪接及其在蛋白质工程中的应用

遗传 ›› 2004, Vol. 26 ›› Issue (2): 249-252.

蛋白质剪接及其在蛋白质工程中的应用

赫冬梅1;2 ; 钱凯先1 ; 沈桂芳1;2 He Dong-Mei1;2; Qian Kai-Xian1; Shen Gui-Fang1;2

1.浙江大学生命科学院,杭州310027；2.中国农业科学院生物技术中心,北京100081 1.College of Biology, Zhejiang University, Hangzhou 310027, China; 2.Biotechnology Research Institute, CAAS, Beijing 100081, China)

收稿日期:1900-01-01 出版日期:2004-03-28 发布日期:2004-03-28

Protein Splicing and Its Application in Protein Engineering

Received:1900-01-01 Online:2004-03-28 Published:2004-03-28

摘要/Abstract

摘要：

蛋白质剪接是蛋白质内含肽介导的，一种在蛋白质水平上翻译后的加工过程，它由一系列分子内的剪切-连接反应组成。蛋白质内含肽是一个蛋白质前体中的多肽序列，可以催化自身从蛋白质前体中断裂,使两侧的蛋白质外显肽连接成成熟的蛋白质。蛋白质内含肽的发现，不仅丰富了遗传信息翻译后加工的理论，在实践中也有广泛的应用前景。Abstract: Protein splicing , which is an intein mediated posttranslational processing, involves a series of intramolecular cleavage-ligation reactions. Intein is an intervening polypeptide which can catalytic self-cleavage from a pre-protein accompanied by the concomitant joining of the two flanking polypeptides (the extein) through a peptide bond. Protein splicing not only enriches genetic theory of posttranslational processing, but also have wide application prospect.

关键词: intein, Protein splicing, 蛋白质外显肽
Key words, 蛋白质内含肽, 蛋白质剪接

赫冬梅，钱凯先，沈桂芳. 蛋白质剪接及其在蛋白质工程中的应用[J]. 遗传, 2004, 26(2): 249-252.

He Dong-Mei, Qian Kai-Xian, Shen Gui-Fang. Protein Splicing and Its Application in Protein Engineering[J]. HEREDITAS, 2004, 26(2): 249-252.