遗传 ›› 2004, Vol. 26 ›› Issue (3): 0-372.

• 论文 •    下一篇

一种新的DNA芯片的封闭方法

何 群 ; 赵雨杰 HE Qun; ZHAO Yu-Jie   

  1. 中国医科大学基础医学院生物芯片中心,沈阳110001Center of Biochip, China Medical University,Shenyang 110001,China
  • 收稿日期:1900-01-01 出版日期:2004-06-10 发布日期:2004-06-10

A New Blocking Method for DNA Chip

  • Received:1900-01-01 Published:2004-06-10 Online:2004-06-10

摘要:



提出了一种新的DNA芯片的封闭方法:1-碘丙烷封闭方法,并对1-碘丙烷和琥珀酸酐两种不同的封闭方法的封闭效果进行了比较实验,研究封闭试剂、封闭时间对氨基DNA芯片杂交结果所产生的影响。结果表明: 1-碘丙烷封闭效果明显好于琥珀酸酐的封闭效果,用1-碘丙烷封闭后的芯片进行杂交,芯片杂交信号强、背景噪声低,灵敏度高,整个封闭过程实验操作简单,时间短,1h即可达到所需效果。1-碘丙烷可以作为一种很好的DNA芯片封闭试剂。Abstract: Suggested a new blocking method for DNA microarray: 1- iodo -propane .we compared the difference between the two block reagents and the blocking results in different blocking time. The results showed that 1-I-prppane is batter-blocking reagent than succinic anhydride in DNA chip.1h blocking time is enough for blocking.

关键词: DNA microarray, 1-碘丙烷, 琥珀酸酐
Key words,
封闭, DNA芯片