摘要：



本研究以实时荧光PCR技术鉴定商业化种植的转基因玉米T14/T25品系。根据转基因玉米T14/T25转入的外源基因质粒图谱，设计转基因引物和探针进行PCR和实时荧光PCR检测，建立了转基因玉米品系鉴定的实时荧光PCR方法。实验结果表明，用TaqMan探针可检测到T14/T25产生的荧光信号，而对其他玉米品系则检测不到荧光信号，为转基因产品的鉴定检测提供了新方法。Abstract: To identify genetically modified (GM) maize T14/T25 lines, a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taqman probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines. The results show that the TaqMan probe could identify T14/T25 maize used, while other GM and NO-GM maize didn’t be detected. The RTF PCR could be a new method for detecting other genetically modified organism.