遗传 ›› 2005, Vol. 27 ›› Issue (1): 30-34.

• 论文 • 上一篇    下一篇

贵州从江侗族Y-DNA及线粒体DNA 序列多态性分析

齐晓岚; 谢 渊; 单可人; 褚 迅; 刘 烜; 李 毅; 赵 艳; 吴昌学; 马 骄; 修 瑾; 任锡麟QI Xiao-Lan;XIE Yuan; SHAN Ke-Ren; CHU Xun; LIU Xuan; LI Yi; ZHAO Yan; WU Chang-Xue; MA Jiao; XIU Jin; Ren Xi-Lin   

  1. 贵阳医学院分子生物学重点实验室, 贵州贵阳 550004) Key Laboratory of Molecular Biology, Guiyang Medical College,Guiyang 550004, China)
  • 收稿日期:1900-01-01 出版日期:2005-01-28 发布日期:2005-01-28

The Sequence Polymorphism of Y-chromosome DNA and mtDNAof Dong Ethnic of Congjiang, Guizhou

  • Received:1900-01-01 Online:2005-01-28 Published:2005-01-28

摘要:

为分析贵州从江侗族父系及母系遗传结构,探讨其起源及迁徒, 通过聚合酶链式反应-限制性片段长度多态性(PCR-RFLP), 研究贵州从江侗族无亲缘关系个体由10个单核苷酸位点(SNPs)组成的Y染色体单倍型及11个单核苷酸位点组成的线粒体DNA单倍群频率。结果显示, 从40份男性样本的Y-SNP基因分型中,得到H6 、H11、H14 共3种单倍型;H11的频率为92.5%;通过对线粒体DNA基因分型,得到6种单倍群,有75%的个体能明确分类其所携带的单倍群特征,说明贵州从江侗族父系遗传构成相对简单。通过主成分分析,证明贵州从江侗族与其他的壮侗语族人群相聚,母系遗传结构复杂,无C单倍群分布可能为该民族特征之一。Abstract: To study the patrilineal and matrilineal genetic structure and the origin of Dong Ethnic of Congjiang Guizhou. Study the distribution of Y-chromosome haplotypes which consisted of 10 SNPs of Y-DNA and mtDNA haplogroups consisted of 11 SNPs by using PCR-RFLP method. The result is three haplotypes H6,H11,H14 were detected, the frequency of H11 is 92.5%. Six haplogroups were identified by mtDNA analysis, 75% of the people can be identified. The patrilineal genetic structure of Dong of Guizhou is simple, Principle component indicated that the structure is closer to Zhuang-Dong branch of Sino-Tibetan language family. The matrilineal genetic structure of Dong of Guizhou is complicated.

关键词: 线粒体DNA单倍型
Key words,
Dong ethnic group, Y-chromosome haplotype, Y染色体单倍型, 贵州侗族