鸡种系嵌和体的研制及其AFLP检测

遗传 ›› 2005, Vol. 27 ›› Issue (3): 367-371.

鸡种系嵌和体的研制及其AFLP检测

胡小芬1;3;谢蓓1;4;于瑞嵩1;2;黄启忠1;张德福1;2;黄路生3;李震1;2

1.上海市农业科学院畜牧兽医研究所，上海，201106;2.上海市农业遗传育种重点实验室，上海，201106;3.江西农业大学江西省动物生物技术重点开放实验室，南昌，330045;4.南京师范大学生命科学学院，南京，210097

收稿日期:2004-06-09 修回日期:2004-09-14 出版日期:2005-06-10 发布日期:2005-06-10
通讯作者: 李震

Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP

HU Xiao-Fen1,3;XIE Bei1,4;YU Rui-Song;HUANG Qi-Zhong;ZHANG De-Fu;HUANG Lu-Sheng;LI Zhen

1. Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106,China ;2.Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences , Shanghai 201106,China;3.Jiangxi Provincial Key Laboratory for Animal Biotechnology,Jiangxi Agricultural University,Nanchang,330045,China;4.School of Life Science,Nanjing Normal University, Nanjing 210097,China

Received:2004-06-09 Revised:2004-09-14 Online:2005-06-10 Published:2005-06-10
Contact: LI Zhen

摘要/Abstract

摘要： 利用密度梯度离心等方法从孵化51-56 h的石歧杂鸡胚血液中提取PGCs，用自制的玻璃注射针将PGCs注射到孵化2.5 d的H系受体鸡胚中制备种系嵌和体鸡；通过筛选AFLP引物建立起家禽嵌和体的AFLP检测方法；经检测20个发育的PGCs受体鸡胚中有８个种系嵌和体，嵌和率为40%。

关键词: 种系嵌和体, 家禽原始生殖细胞, 扩增片段长度多态性

Abstract: PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.

胡小芬，谢蓓，于瑞嵩，黄启忠，张德福，黄路生，李震. 鸡种系嵌和体的研制及其AFLP检测[J]. 遗传, 2005, 27(3): 367-371.

HU Xiao-Fen, XIE Bei, YU Rui-Song, HUANG Qi-Zhong, ZHANG De-Fu, HUANG Lu-Sheng, LI Zhen. Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP[J]. HEREDITAS, 2005, 27(3): 367-371.

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