流式细胞术分析和分拣植物染色体

遗传 ›› 2005, Vol. 27 ›› Issue (3): 461-465.

流式细胞术分析和分拣植物染色体

李立家;宋运淳

武汉大学生命科学学院植物发育生物学教育部重点实验室，武汉430072

收稿日期:2004-08-20 修回日期:2004-10-09 出版日期:2005-06-10 发布日期:2005-06-10
通讯作者: 李立家

Flow Cytometric Analysis and Sorting of Plant Chromosomes

LI Li-Jia;SONG Yun-Chun

Wuhan University,Key Laboratory of the Ministry of Education of Plant Developmental Biology,Wuhan 430072,China

Received:2004-08-20 Revised:2004-10-09 Online:2005-06-10 Published:2005-06-10
Contact: LI Li-Jia

摘要/Abstract

摘要： 流式细胞术是当染色体、细胞核和细胞等颗粒随着流动的液体（水或缓冲液）通过一个测量点时，被探测器探测到，这样根据颗粒的物理和化学特征而将不同的颗粒分开并计数分拣的技术。流式细胞分析在人类基因组计划中发挥了重要作用，流式细胞技术的应用也适用于植物，目前这个技术应用范围包括流式核型分析，分拣纯化染色体，定位基因，构建文库等。文章综述了流式细胞术在植物基因组分析方面的研究进展。

关键词: 染色体分拣, 染色体专性文库, 流式细胞仪, 植物染色体流式核型

Abstract: Flow cytometry refers to the technique where the measurement of the physical and chemical characteristics of particles including chromosomes, nuclei and cells is made as the particles pass in a fluid stream through a measuring point surrounded by an array of detectors. FCM (flow cytometry) analysis has played an important role in human genome projects. This technique is now increasingly used to establish flow karyotypes, sort chromosomes, map genes and construct DNA libraries in plants. This paper reviewed progress in flow cytometric analysis of plant genomes.

中图分类号:

Q942

李立家，宋运淳. 流式细胞术分析和分拣植物染色体[J]. 遗传, 2005, 27(3): 461-465.

LI Li-Jia, SONG Yun-Chun. Flow Cytometric Analysis and Sorting of Plant Chromosomes[J]. HEREDITAS, 2005, 27(3): 461-465.