无载体框架序列转基因小麦中外源1Ax1基因表达框的遗传分析

遗传 ›› 2006, Vol. 28 ›› Issue (6): 695-698.

无载体框架序列转基因小麦中外源1Ax1基因表达框的遗传分析

姚琴,丛玲, 汪越胜,陈明洁,杨广笑,何光源

华中科技大学中英HUST-RRes作物遗传工程和基因组学联合实验室，武汉 430074

收稿日期:2005-10-16 修回日期:2006-01-16 出版日期:2006-06-10 发布日期:2006-06-10
通讯作者: 何光源

Inheritance of transgene 1Ax1 in transgenic wheat (Triticum aestivum L.) with linear transgene lacking vector backbone sequence

YAO Qin, CONG Ling, WANG Yue-Sheng, CHEN Ming-Jie,YANG Guang-Xiao, HE Guang-Yuan

China-UK HUST-RRes Genetic Engineering and Genomics Joint Lab, Huazhong University of Science and Technology (HUST), Wuhan 430074, China

Received:2005-10-16 Revised:2006-01-16 Online:2006-06-10 Published:2006-06-10
Contact: HE Guang-Yuan

摘要/Abstract

摘要： 为了研究无载体框架序列转基因小麦中转基因表达框的遗传规律，选育稳定表达的转基因株系，利用基因枪介导1Ax1基因的最小表达框转化得到了转基因小麦，对其后代转基因植株中1Ax1基因表达框的遗传进行了分析，结果表明：无载体框架结构的1Ax1基因在转基因后代中稳定遗传，在T1代中呈现3:1的分离，遵从孟德尔遗传模式；SDS－PAGE分析表明其中部分转基因后代分离出新的高分子量蛋白亚基分子量略低于1Dx5亚基。

关键词: 载体框架结构, 转寄因小麦, 1Ax1基因, 高分子量麦谷蛋白亚基

Abstract: To analysis identify the heritance of transgene minimal expression cassette in transgenic plant and to find the stable transgenic lines, transgenic wheat was regenerated with linear transgene 1Ax1 lacking vector backbone sequence via particle bombardment, consecutive generations of which have been investigated to explore the heredity of transgene 1Ax1 cassetts expression. The results showed that linear transgene 1Ax1 expression cassette was stably expressed and segerated at the rate of 3 to 1 in T1 generation, suggesting the functional copies of transgene 1Dx5 integrated in one locus, abiding by Mendelian genetic law. SDS-PAGE analysis indicated that parts of transgenic lines had a new HMW glutenin band of an apparent Mr lower than that of the subunit 1Dx5.

中图分类号:

Q943.2

姚琴，丛玲，汪越胜，陈明洁，杨广笑，何光源. 无载体框架序列转基因小麦中外源1Ax1基因表达框的遗传分析[J]. 遗传, 2006, 28(6): 695-698.

YAO Qin, CONG Ling, WANG Yue-Sheng, CHEN Ming-Jie, YANG Guang-Xiao, HE Guang-Yuan. Inheritance of transgene 1Ax1 in transgenic wheat (Triticum aestivum L.) with linear transgene lacking vector backbone sequence[J]. HEREDITAS, 2006, 28(6): 695-698.