大鳞副泥鳅3个Dmrt基因DM保守区的序列分析

遗传 ›› 2008, Vol. 30 ›› Issue (11): 1448-1452.

大鳞副泥鳅3个Dmrt基因DM保守区的序列分析

徐玲花¹;严镇钧²;曾庆韬³

1. 黄石理工学院化学与材料工程学院, 湖北黄石 435003;
2. 湖北师范学院生命科学学院, 湖北黄石 435003;
3. 湖北大学生命科学学院, 武汉 430062

收稿日期:2007-12-20 修回日期:2008-03-08 出版日期:2008-11-10 发布日期:2008-11-10
通讯作者: 曾庆韬

Sequence analysis of the DM domain of three Dmrt genes in loach, Paramisgurnus dabryanus

XU Ling-Hua¹;YAN Zhen-Jun²;ZENG Qing-Tao³

1. School of Chemical and Material Engineering, Huangshi Institute of Technology, Huangshi Hubei Province, 435003, China;
2. College of Life Science, Hubei Normal University, Huangshi Hubei Province, 435003, China;
3. College of Life Science, Hubei University, Wuhan 430062, China

Received:2007-12-20 Revised:2008-03-08 Online:2008-11-10 Published:2008-11-10
Contact: ZENG Qing-Tao

摘要/Abstract

摘要： 摘要: 利用简并PCR克隆技术, 扩增和克隆了大鳞副泥鳅(Paramisgurnus dabryanus)Dmrt基因的DM结构域, 获得了3个具有不同DM序列的克隆。结果表明, 在大鳞副泥鳅基因组中存在Dmrt基因家族的多个成员。同源性比较和系统进化分析显示不同进化地位脊椎动物的Dmrt基因存在高度的进化保守性

关键词: Dmrt基因, DM结构域, 大鳞副泥鳅

Abstract: Abstract: We amplified genomic DNA of the Paramisgurnus dabryanus using the DM degenerate primers and detected a band, approximately 150 bp. After cloned into pMD18-T vector and sequenced, three sequences showed high homology with the DM domain. They were named as PdDmrt1, PdDmrt3, and PdDmrt5. Based on similarities of amino acid se-quences of the DM domain, Dmrt gene protein sequences from six vertebrates species were included in a phylogenetic tree.

徐玲花，严镇钧，曾庆韬. 大鳞副泥鳅3个Dmrt基因DM保守区的序列分析[J]. 遗传, 2008, 30(11): 1448-1452.

XU Ling-Hua, YAN Zhen-Jun, ZENG Qing-Tao. Sequence analysis of the DM domain of three Dmrt genes in loach, Paramisgurnus dabryanus[J]. HEREDITAS, 2008, 30(11): 1448-1452.

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