辣椒31个优良自交系的亲本类群分析

遗传 ›› 2008, Vol. 30 ›› Issue (2): 237-237―245.

辣椒31个优良自交系的亲本类群分析

任羽^1,2, 张银东³, 尹俊梅¹, 王得元²

1. 中国热带农业科学院热带作物品种资源研究所, 儋州 571737;
2. 广东省农业科学院蔬菜研究所, 广州 510640;
3. 中国热带农业科学院热带作物生物技术国家重点实验室, 海口 571101

收稿日期:2007-07-11 修回日期:2007-11-01 出版日期:2008-02-10 发布日期:2008-02-10
通讯作者: 任羽

Parent grouping of 31 elite inbred lines in hot pepper (Capsicum annuum L.)

REN Yu^1,2, ZHANG Yin-Dong ³, YIN Jun-Mei¹, WANG De-Yuan ²

1. Tropical Crops Genetic Resources Institute, CATAS, Danzhou 571737, China;
2. Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
3. State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou 571101, China

Received:2007-07-11 Revised:2007-11-01 Online:2008-02-10 Published:2008-02-10
Contact: Ren Yu

摘要/Abstract

摘要：

以包含我国重要尖椒品种的亲本材料在内的31份优良自交系为材料, 利用SRAP标记和基因型值分析技术开展了辣椒自交系间遗传差异的分析与类群划分研究。结果表明: 在30个引物组合中, 27个引物组合可以在自交系间扩增出多态性条带, 共扩增出310个多态性条带, 平均每个引物组合产生11.5个多态性条带, 显示出SRAP技术具有较强的分析效率; 基于SRAP标记和Yule相似系数对这些自交系进行的聚类分析中, 可以基本区分辣椒的2个变种(C. annuum var. grossum和C. annuum var. longum), 而且可以反映出自交系间的亲缘及系谱关系; 在相似系数为0.67处, 可将这31个自交系分为4个类群; 基于基因型值和标准Euclidean距离对这些自交系进行的聚类分析可成功地将辣椒的两个变种完全区分; 在遗传距离约4.5处, 可将这31个自交系分为4个类群; 自交系间基于SRAP标记与基因型值的遗传距离存在一定的相关性。

关键词: 辣椒, 优良自交系, SRAP标记, 基因型值分析, 亲本类群

Abstract:

Genetic differences were examined among thirty-one elite inbred lines in Capsicum annuum L. Two types of analytic technologies, i.e. SRAP markers and genotypes of traits, were used, and their relative effectiveness was compared. 27 of 30 primer combinations could amplify 310 polymorphic bands among inbred lines, indicating SRAP marker was efficient to detect polymorphism among pepper inbred lines. A dendrogram of 31 inbred lines based on SRAP markers and Yule coefficients could basically separate lines of C. annuum var. grossum and C. annuum var. longum, and reveal the pedigrees of inbred lines. A dendrogram of 31 inbred lines based on genotypes of traits and standardized Euclidean coefficients could separate lines of C. annuum var. grossum and C. annuum var. longum. The SRAP marker genetic distances were correlated with distances based on the genotypes of traits. These results and their application in the development of hot pepper F1 hy-brids were also discussed.

任羽，张银东，尹俊梅，王得元. 辣椒31个优良自交系的亲本类群分析[J]. 遗传, 2008, 30(2): 237-237―245.

REN Yu, ZHANG Yin-Dong, YIN Jun-Mei, WANG De-Yuan. Parent grouping of 31 elite inbred lines in hot pepper (Capsicum annuum L.)[J]. HEREDITAS, 2008, 30(2): 237-237―245.