遗传 ›› 2008, Vol. 30 ›› Issue (5): 620-626.doi: 10.3724/SP.J.1005.2008.00620

• 研究报告 • 上一篇    下一篇

拟南芥TCH4基因启动区转录调控元件的计算识别

张长青1,2,3, 王进1, 高翔2   

  1. 1. 南京大学医药生物技术国家重点实验室, 南京 210093;
    2. 南京大学模式动物研究所, 南京 210061;
    3. 金陵科技学院园艺学院, 南京 210038

  • 收稿日期:2007-10-22 修回日期:2007-11-29 出版日期:2008-05-10 发布日期:2008-05-10
  • 通讯作者: 王进

Computational identification of transcriptional regulatory elements in Arabidopsis TCH4 promoter

ZHANG Chang-Qing1,2,3, WANG Jin1, GAO Xiang2   

  1. 1. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China;
    2. Model Animal Research Center, Nanjing University, Nanjing 210061, China;
    3. School of Horticulture, Jinling Institute of Technology, Nanjing 210038, China
  • Received:2007-10-22 Revised:2007-11-29 Online:2008-05-10 Published:2008-05-10
  • Contact: WANG Jin

摘要:

TCH4基因在植物次生生长、疾病抵抗和逆境适应方面具有重要作用, 能被多种激素、环境和机械信号诱导表达。利用拟南芥TCH4的直系同源基因和芯片数据进行了启动子序列分析, 结果共识别出9个转录调控元件。它们均包含有已知元件序列, 并且在部分共表达基因和对应的直系同源基因启动子中排列顺序一致。根据已有TCH4基因启动子研究, 其中4个已被报道, 另5个为本研究新发现。根据预测结果进行知识整合, 构建了TCH4基因转录调控机制模型。

关键词: 生物信息学, 转录调控元件, TCH4基因

Abstract:

Arabidopsis TCH4 gene plays an important role in the biological processes related to plant secondary growth, resistance to pathogen, and adaptation to environmental stresses. It is up-regulated by various hormonal, environmental, and mechanical stimuli. Here, we identified 9 transcriptional regulatory elements from TCH4 promoter by bioinformatics approach. In which, 4 elements have been reported previously, and 5 elements are newly identified in this study. All of the identified elements contain the sequences of known cis-elements. Especially, their distribution along some co-expressed gene promoters and the orthologous promoters is typically clustered and syntenic. Based on our predictions and the information of known cis-elements, a model representing the transcriptional regulation mechanism was proposed for TCH4 gene in re-sponse to hormonal, mechanical, and environmental stimuli.