遗传 ›› 2005, Vol. 27 ›› Issue (6): 915-918.

• 研究报告 • 上一篇    下一篇

朱鹮随机微卫星扩增多态DNA(RMAPD)研究

张永德1;范光丽1;雷初朝1;孙维斌1;蓝贤勇1;陈 宏1;2   

  1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌 712100
  • 收稿日期:2004-11-16 修回日期:2005-01-21 出版日期:2005-12-10 发布日期:2005-12-10
  • 通讯作者: 范光丽

Research on the Genetic Polymorphism in Crested Ibis by RMAPD

ZHANG Yong-De1, FAN Guang-Li1,LEI Chu-Zhao1, SUN Wei-Bin1, LAN Xian-Yong1, CHEN Hong1,2   

  1. College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China
  • Received:2004-11-16 Revised:2005-01-21 Online:2005-12-10 Published:2005-12-10
  • Contact: FAN Guang-Li

摘要: 首次利用RMAPD标记对43只洋县人工饲养朱鹮群体的遗传多态性进行了分析。结果表明,RMAPD技术的稳定性与多态性比RAPD高。12对引物共扩增出2147条带纹,93条具有多态性。朱鹮的条带共享率为0.718,遗传多样性指数为3.664,反映群体内个体间相似程度较高,遗传多态性较低,需要加强对朱鹮的遗传多样性保护。

关键词: 遗传多态性, 朱鹮, RMAPD

Abstract: The genetic polymorphism of Yangxian artificially reared 43 crested ibises (Nipponia nippon) was firstly investigated by RMAPD (random microsatellite amplify polymorphic DNA) marker. The results showed that RMAPD was more stable and polymorphic than RAPD. 2147 bands were amplified by 12 pairs of primers,of which 93 bands had polymorphisms. The band sharing frequency and the genetic diversity index were 0.718 and 3.664, respectively, indicating that the genetic structure of crested ibis population was simple and poor genetic variations existed in crested ibis population in Yangxian. It is imperious to protect the genetic diversity of crested ibis population.

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