遗传 ›› 2005, Vol. 27 ›› Issue (6): 919-924.

• 研究报告 • 上一篇    下一篇

凡纳滨对虾微卫星位点在两个选育家系中遗传的初步研究

张留所1;2; 相建海1   

  1. 中国科学院海洋研究所 实验海洋学重点实验室 青岛 266071
  • 收稿日期:2005-01-21 修回日期:1900-03-01 出版日期:2005-12-10 发布日期:2005-12-10
  • 通讯作者: 相建海

A Preliminary Study on the Inheritance of Microsatellite in Two Selective Breeding Families of Shrimp ( Litopenaeus vannamei)

ZHANG Liu-Suo1 2, XIANG Jian-Hai1   

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  • Received:2005-01-21 Revised:1900-03-01 Online:2005-12-10 Published:2005-12-10
  • Contact: XIANG Jian-Hai

摘要: 利用两个选育凡纳滨对虾全同胞家系研究了10个微卫星位点的遗传特征。通过ABI310或3100测序仪检测, 在所观察到的20个基因型比例(genotypic ratios)(10个微卫星位点 X 2个家系)中,有17个基因型比例符合孟德尔遗传。微卫星位点TUMXLv8.220在两个家系中均存在无效等位基因,从而3个不符合孟德尔遗传基因型中2个可由无效等位基因来解释。TUMXLv 3.1在06家系偏离了1:1:1:1的孟德尔预期比。3个微卫星位点(TUMXLv5.66,TUMXLv7.74,TUMXLv8.224)在两个家系中均表现单态。3个微卫星位点(TUMXLv5.45,TUMXLv7.56,TUMXLv8.256)在两个家系均既表现多态又遵循孟德尔共显性遗传, 是亲子鉴定和种群遗传分析的较好选择。结果显示在应用微卫星标记进行遗传分析之前利用全同胞家系进行遗传模式研究是非常必要的。

关键词: 无效等位基因, 孟德尔遗传, 微卫星, 凡纳滨对虾

Abstract: Two full-sib families of Litopenaeus vannamei were used to study the inheritance of allelic variation at 10 microsatellite loci. Of the 20 genotypic ratios observed (10 loci x 2 crosses), 17 ratios conformed to Mendelian segregation. When null alleles were considered, one loci (TUMXLv8.220) confirmed Mendelian expectations in all families. While one loci (TUMXLv3.1) showed deviation from family 06. Three loci (TUMXLv5.66,TUMXLv7.74,TUMXLv8.224)were monomorphic in both controlled crosses; 3 loci were polymorphic and confirmed to Mendelian ratios in all families, can be used for parental analysis and population genetic studies. These results indicated the need to test the inheritance pattern for microsatellite markers in shrimp before using them for population genetic or kinship analysis.

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