遗传 ›› 2006, Vol. 28 ›› Issue (2): 165-170.

• 研究报告 • 上一篇    下一篇

我国主要地方绵羊品种mtDNA D-loop区PCR-RFLP研究

李祥龙1;2;张增利;巩元芳2;刘铮铸2;贾 青1;王立泽1   

  1. 1.河北农业大学动物科技学院,保定 071001;2. 河北科技师范学院动物科学系,昌黎 066600
  • 收稿日期:2005-02-25 修回日期:2005-04-17 出版日期:2006-02-10 发布日期:2006-02-10
  • 通讯作者: 李祥龙

Study on mtDNA D-loop of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP

LI Xiang-Long1,ZHANG Zeng-Li1,GONG Yuan-Fang2,LIU Zheng-Zhu2,JIA Qing1,WANG Li-Ze1
  

  1. 1. Department of Animal Science & Technology, Hebei Agriculture University, Baoding,071001,China 2. Department of Animal Science, Hebei Normal University of Science & Technology, Changli, 066600, China
  • Received:2005-02-25 Revised:2005-04-17 Online:2006-02-10 Published:2006-02-10
  • Contact: XiangLong Li

摘要:

利用5种限制性内切酶(Hinf I,Msp I,Sau3A I,Xsp I,Taq I),采用PCR-RFLP技术研究了我国9个地方绵羊品种以及2个引入品种共计83只绵羊个体线粒体DNA D-loop区的多态性。结果表明,我国主要地方绵羊品种线粒体DNA D-loop区存在两种基本单体型,提示我国主要地方绵羊品种起源于两个母系祖先。线粒体DNA D-loop区多态度为0.042 1%,说明我国地方绵羊品种线粒体DNA多态度较为贫乏。

 
 

关键词: D-loop, PCR-RFLP, 线粒体DNA, 地方绵羊品种

Abstract: The polymorphism of mtDNA D-loop of 83 individuals from 9 Chinese indigenous sheep breeds and 2 imported sheep breeds were studied with 5 endonucleases, Hinf I,Msp I,Sau3A I,Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The averaged polymorphic degree (π value=0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.

 

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