遗传 ›› 2008, Vol. 30 ›› Issue (12): 1621-1628.doi: 10.3724/SP.J.1005.2008.01621

• 研究报告 • 上一篇    下一篇

西伯利亚蓼PsPIP1基因的克隆及其在NaHCO3胁迫下的表达

王垠; 刘关君; 阎秀峰; 杨传平; 刘明坤; 曲春浦

  

  1. 东北林业大学林木遗传育种与生物技术教育部重点实验室, 哈尔滨150040

  • 收稿日期:2008-04-21 修回日期:2008-07-14 出版日期:2008-12-10 发布日期:2008-12-10
  • 通讯作者: 刘关君

Cloning of PsPIP1 Gene from Polygonum sibiricum Laxm. and the analysis of expression in NaHCO3 stress.

WANG Yin; LIU Guan-Jun; YAN Xiu-Feng; YANG Chuan-Ping; LIU Ming-Kun;
QU Chun-Pu

  

  1. The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University,
    Harbin
    150040, China
  • Received:2008-04-21 Revised:2008-07-14 Online:2008-12-10 Published:2008-12-10
  • Contact: LIU Guan-Jun

摘要:

应用cDNA末端快速扩增(RACE)技术从西伯利亚蓼叶cDNA文库中克隆了质膜内在蛋白基因(PsPIP1)的完整编码区cDNA序列(GenBank accession No. EU626398), 长度为1 004 bp, 编码285个氨基酸。基于和其他植物水通道蛋白的氨基酸序列、推测的三维结构的比较以及系统进化分析结果, 初步确定此基因为水通道蛋白基因家族中的PIP1亚族成员。RT-PCR结果显示, PsPIP1在西伯利亚蓼的地下茎、茎、叶中均有表达, 叶中表达量最高, 地下茎次之, 茎中最低。在NaHCO3胁迫与去胁迫的过程中, 此基因在地下茎、茎、叶中的表达模式也有较明显的差异。

关键词: 实时定量RT-PCR, 基因克隆, 西伯利亚蓼, 水通道蛋白

Abstract:

Abstract: Gene PsPIP1 (GenBank accession No. EU626398) containing a complete ORF was obtained using rapid ampli-fication of cDNA ends (RACE) from the cDNA library of Polygonum sibiricum Laxm. leaves. The length of cDNA was 1 004 bp, which encoded a peptide of 285 amino acid residues. Based on other kinds of plant aquaporin amino acid sequences, the phylogenetic evolution, and tertiary structure of protein comparison, this gene was classified into aquaporin subfamily. Expression analysis by Real-time PCR showed that PsPIP1 gene was expressed in leaves, stems, and underground stems. The expression level of PsPIP1 gene was higher in leaves than in underground stems and was the lowest in stems. The ex-pression pattern of PsPIP1 gene induced by NaHCO3 stress and de-stressing also varied remarkably.