微卫星座位与丝羽乌骨鸡蛋用系开产性状相关分析

遗传 ›› 2008, Vol. 30 ›› Issue (2): 203-203―208.

微卫星座位与丝羽乌骨鸡蛋用系开产性状相关分析

康丽^{1, 2}, 朱庆¹

1. 四川农业大学动物科技学院, 雅安 625014;
2. 山东农业大学动物科技学院, 泰安 271018

收稿日期:2007-07-04 修回日期:2007-11-01 出版日期:2008-02-10 发布日期:2008-02-10
通讯作者: 康丽

The analysis of Correlation Between Microsatellite DNA markers and start laying traits in Silkies Laying Breeds

KANG Li^{1, 2}, ZHU Qing¹

1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China;
2. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China

Received:2007-07-04 Revised:2007-11-01 Online:2008-02-10 Published:2008-02-10

摘要/Abstract

摘要：

采用多重PCR扩增结合荧光标记全自动电泳检测技术, 对鸡基因组1号染色体和Z染色体上20个微卫星座位与丝羽乌骨鸡BM、BF两个蛋用新品系180个个体的开产性状进行相关性分析。结果表明: 位于1号染色体上的MCW0068、MCW0200与开产蛋重相关显著(P<0.05); 位于1号染色体上的MCW0068、MCW0208和位于Z染色体上的MCW0128与开产体重相关显著(P<0.05); 而位于Z染色体上的MCW0154与开产体重相关极显著(P<0.01)。

关键词: 多重PCR, 开产性状, 微卫星标记, 丝羽乌骨鸡

Abstract:

Twenty microsatellite loci on Chromosome 1 and Chromosome Z were selected from chicken genome for correlation analysis with traits at first laying in silkies. The 180 silkies were from two egg-laying lines of BM and BF. Genotyping of microsatellite loci was carried out by using fluorescein-labeled multiplex-PCR detection technology. The results showed that MCW0068 and MCW0200 on Chromosome 1 were significantly associated with egg weight at first laying (P<0.05), MCW0068 and MCW0208 on Chromosome 1 and MCW0128 on Chromosome Z were significantly associated with body weight at first laying (P<0.05), and MCW0154 on Chromosome Z was highly significantly associated with body weight at first laying (P<0.01).

康丽，朱庆. 微卫星座位与丝羽乌骨鸡蛋用系开产性状相关分析[J]. 遗传, 2008, 30(2): 203-203―208.

KANG Li, ZHU Qing. The analysis of Correlation Between Microsatellite DNA markers and start laying traits in Silkies Laying Breeds[J]. HEREDITAS, 2008, 30(2): 203-203―208.

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