遗传 ›› 2009, Vol. 31 ›› Issue (7): 755-762.doi: 10.3724/SP.J.1005.2009.00755

• 研究报告 • 上一篇    下一篇

白菜抽薹性状相关基因的cDNA-AFLP分析

邹艳敏1, 2;于拴仓2;张凤兰2;余阳俊2;赵岫云2;张德双2   

  1. 1. 首都师范大学生命科学学院, 北京 100037;
    2. 北京农林科学院蔬菜研究中心, 北京 100097
  • 收稿日期:2008-11-13 修回日期:2009-01-13 出版日期:2009-07-10 发布日期:2009-07-10
  • 通讯作者: 张凤兰

cDNA-AFLP analysis on transcripts associated with bolting in Brassica rapa L. ssp. pekinensis

ZOU Yan-Min1, 2;YU Shuan-Cang2;ZHANG Feng-Lan2;YU Yang-Jun2;ZHAO Xiu-Yun2;ZHANG De-Shuang2   

  1. 1. College of Life Science, Capital Normal University, Beijing 100037, China;
    2. Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing Vegetable Research Center, Beijing 100097, China
  • Received:2008-11-13 Revised:2009-01-13 Online:2009-07-10 Published:2009-07-10
  • Contact: ZHANG Feng-Lan

摘要: 先期抽薹严重制约着我国春大白菜及高原或高海拔地区的春夏白菜栽培, 给生产造成重大损失, 因而研究白菜作物低温春化途径抽薹和开花的相关基因对白菜育种具有重要意义。文章选取极早抽薹株系DH-54和极晚抽薹株系DH-43为材料, 利用256对引物组合进行cDNA-AFLP分析, 筛选到与抽薹开花相关的差异表达的转录衍生片段(Transcript-derived fragment, TDF)191条, 对其中82个TDF进行克隆测序。结果表明: 52个TDF与NCBI或TIGR已有序列同源, 功能涉及代谢、胁迫及逆境应答、信号转导、转录调控等等; 22个TDF在NCBI或TIGR找到同源序列, 但功能未知; 有8个TDF在NCBI或TIGR找不到同源序列, 可能是一些新基因。

关键词: 抽薹, cDNA-AFLP, 春化, 大白菜(Brassica rapa L. ssp. pekinensis)

Abstract: Premature bolting, caused by low temperature in spring and summer cultivation in low land and high land re-spectively, leads to reduction of the yield and quality of the harvested products in Chinese cabbage. Therefore, exploring genes involved in vernalization response is important to the improvement of Chinese cabbage varieties. Here, one extremely early bolting line (DH-54) and one extremely late bolting line (DH-43) were employed, and the cDNA-AFLP approach was used to identify key components involved in the low-temperature required vernalization response. Of 256 primer recombi-nations screened, a total of 191 differential expressed transcript-derived fragments (TDFs) were identified, and 82 TDFs were sequenced. BLAST and alignments showed that 52 candidate TDFs shared high levels of similarity with genes of known function, 22 TDFs of unknown function and 8 novel ESTs. The TDFs of known function were involved in genes encoding enzymes working in metabolism, proteins related to stress and defense, signal transduction, and transcription regulation, etc.