ApoB与UCP基因间上位效应对鸡腹脂性状影响的遗传学分析

doi:10.3724/SP.J.1005.2010.00059

遗传 ›› 2010, Vol. 32 ›› Issue (1): 59-66.doi: 10.3724/SP.J.1005.2010.00059

ApoB与UCP基因间上位效应对鸡腹脂性状影响的遗传学分析

户国, 王守志, 张森, 陈维星, 刘爽, 田建伟, 李辉

东北农业大学动物科学技术学院, 哈尔滨 150030

收稿日期:2009-05-21 修回日期:2009-09-28 出版日期:2010-01-20 发布日期:2010-01-15
基金资助:
国家高技术研究发展计划项目(863计划) (编号：2006AA10A120), 国家重点基础研究发展规划(973计划)项目(编号：2006CB102105)和黑龙江省教育厅项目(编号：11531025)资助

Genetic analysis of epistatic effects between ApoB and UCP on abdominal fat trait in chicken

HU Guo, WANG Shou-Zhi, ZHANG Sen, CHEN Wei-Xing, LIU Shuang, TIAN Jian-Wei, LI Hui

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received:2009-05-21 Revised:2009-09-28 Published:2010-01-20 Online:2010-01-15

摘要/Abstract

摘要：

已有研究表明, 上位效应在畜禽重要复杂经济性状的表型形成过程中发挥重要作用。文章选取对肉鸡7 周龄腹脂率有显著影响的载脂蛋白B(Apoliprotein B, ApoB)基因T123G位点与解耦连蛋白(Uncoupling protein, UCP)基因C1197A位点, 在东北农业大学肉鸡高、低腹脂双向选择品系8、9、10世代内检测这两个SNPs的多态性并利用Natural and Orthogonal InterActions (NOIA)模型分析二者之间的上位效应对7周龄腹脂率的影响。结果表明, 在高脂系内这两个位点之间存在着对7周龄腹脂率有显著影响的上位效应组分(P<0.05), 并且在连续多世代选育过程中仍能持续稳定存在; 同时, 在低脂系中二者之间各上位效应组分对腹脂率均无影响(P>0.05)。此结果意味着, 至少在高脂系内, 腹脂率的遗传受到这两个基因间上位效应的影响; 同时提示两系间脂肪性状QTL或重要候选基因功能位点之间不同的遗传互作模式可能是引起这两个品系腹脂性状巨大表型差异的重要影响因素之一。

关键词: 鸡, 脂肪, 基因互作, ApoB, UCP, SNP

Abstract:

It has been found that epistasis for selective response plays an indispensible role in animal genetics and breeding. In this study, the polymorphisms of T123G in apoliprotein B (ApoB) and C1197A in uncoupling protein (UCP) among individuals from the 8th to the 10th generation populations of the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHFL) were detected, and genetic analysis of the epistatic effects between the two SNPs on abdominal fat percentage (AFP) was performed using Natural and Orthogonal InterActions (NOIA) model. According to these assays, we concluded that at least one out of four epistatic components between these two SNPs was significantly associated with AFP (P<0.05) in fat lines from the 8th to the 10th generations of NEAUHFL; on the contrary, none was significantly associated with AFP (P>0.05) in lean lines. Our results suggested that epistatic interactions among QTLs and functional SNPs in candidate genes affecting fat traits might lead to differences in growth patterns of fat traits between lean and fat chicken lines.

Key words: chicken, fat, geneinteraction, ApoB, UCP, SNP

户国，王守志，张森，陈维星，刘爽，田建伟，李辉. ApoB与UCP基因间上位效应对鸡腹脂性状影响的遗传学分析[J]. 遗传, 2010, 32(1): 59-66.

HU Guo, WANG Shou-Zhi, ZHANG Sen, CHEN Wei-Xing, LIU Shuang, TIAN Jian-Wei, LI Hui. Genetic analysis of epistatic effects between ApoB and UCP on abdominal fat trait in chicken[J]. HEREDITAS, 2010, 32(1): 59-66.

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ApoB与UCP基因间上位效应对鸡腹脂性状影响的遗传学分析

Genetic analysis of epistatic effects between ApoB and UCP on abdominal fat trait in chicken

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