遗传 ›› 2011, Vol. 33 ›› Issue (3): 255-261.doi: 10.3724/SP.J.1005.2011.00255

• 研究报告 • 上一篇    下一篇

澜沧江老挝纹胸鮡Cyt b基因的序列变异与遗传结构分析

金菊1, 2, 刘明典2, 3, 阴双雨1,2, 汪登强2, 刘绍平2, 陈大庆2, 4   

  1. 1. 华中农业大学水产学院, 武汉 430070 2. 中国水产科学研究院长江水产研究所, 荆州 434000 3. 西南大学生命科学学院, 重庆 400715 4. 中国水产科学研究院淡水渔业研究中心, 无锡 214081
  • 收稿日期:2010-07-10 修回日期:2010-09-28 出版日期:2011-03-20 发布日期:2011-02-25
  • 通讯作者: 刘绍平 E-mail:lsp@ yfi.ac.cn
  • 基金资助:

    国家环保部项目资助

Genetic structure and variation of Glyptothorax laosensis of the Lancang River based on cytochrome b

JIN Ju1, 2, LIU Ming-Dian2, 3, YIN Shuang-Yu1, 2, WANG Deng-Qiang2, LIU Shao-Ping2, CHEN Da-Qing2, 4   

  1. 1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China 2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China 3. College of Life Science, Southwest University, Chongqing 400715, China 4. Freshwater fishery research center of Chinese Academy of Fishery Sciences, Wuxi 214081, China
  • Received:2010-07-10 Revised:2010-09-28 Online:2011-03-20 Published:2011-02-25
  • Contact: LIU Shao-Ping E-mail:lsp@ yfi.ac.cn

摘要: 老挝纹胸鮡是分布于澜沧江流域的一种特有鱼类, 喜激流底栖生活。为了解其各地理种群间的遗传结构与遗传变异, 文章分析了129尾采自澜沧江8条支流水系的老挝纹胸鮡mtDNA细胞色素b基因1 138 bp的序列变异, 共发现16个多态性位点, 定义了15个单倍型。澜沧江老挝纹胸鮡遗传变异较低, 平均单倍型多样性指数和核苷酸多样性指数分别是h=0.299, π=0.299, 有3个种群甚至没有变异, 分子变异方差分析结果表明各种群内变异大于各种群间变异, 种群间没有差异。对老挝纹胸鮡所有种群进行的单倍型错配分布呈现单峰型, 中性检验结果均为负值(Tajima's D=-2.36965, P<0.02; Fu's Fs=-20.975, P<0.05)表明, 澜沧江老挝纹胸鮡自然种群可能经历过近期的种群扩张事件。

关键词: 老挝纹胸鮡, mtDNA Cyt b, 遗传结构, 种群扩张

Abstract: The hillstream catfish, Glyptothorax laosensis, is endemic to basin of Lancang River (also known as Mekong River). Genetic variation was detected within and among 8 populations of G. laosensis in Lancang River based on mitochondrial cytochrome b sequences. A total 16 nucleotides sites were detected in 1 138 bp fragment, which defined 15 haplotypes from 129 sequences. The variation level was very low, with mean h=0.299 and π=0.00032, respectively, and no variation was detected in some populations. Hierarchical analysis of molecular variance (AMOVA) showed no significant genetic structuring among populations. The unimodel mismatch distribution of pairwise haplotype difference and the negatively selective neutrality test (Tajima's D=-2.3696, P<0.02; Fu's Fs=-20.975, P<0.05) suggested that a recent population expansion had occurred.

Key words: Glyptothorax laosensis, mtDNA Cyt b, genetic diversity, population expansion