遗传 ›› 2011, Vol. 33 ›› Issue (12): 1317-1326.doi: 10.3724/SP.J.1005.2011.01317

• 综述 • 上一篇    下一篇

新一代测序技术在植物转录组研究中的应用

梁烨, 陈双燕, 刘公社   

  1. 中国科学院植物研究所资源植物研发重点实验室, 北京 100093
  • 收稿日期:2010-11-16 修回日期:2011-08-24 出版日期:2011-12-20 发布日期:2011-12-25
  • 通讯作者: 陈双燕 E-mail:sychen@ibcas.ac.cn
  • 基金资助:

    国家重点基础研究发展计划项目(“973”计划)(编号:2007CB108905)资助

Application of next generation sequencing techniques in plant transcriptome

LIANG Ye, CHEN Shuang-Yan, LIU Gong-She   

  1. The Research and Development Laboratory for Plant Resources, Institute of Bontany, Chinese Acdemy of Sciences, Beijing 100093, China
  • Received:2010-11-16 Revised:2011-08-24 Online:2011-12-20 Published:2011-12-25

摘要: 随着DNA测序技术的发展, 新一代测序技术以其高通量、低成本的特点, 成为越来越多的生物学研究者在开展工作时的首选。在所有的新一代测序技术中, 454测序系统是最早实现商业化且发展相对成熟的一种, 目前被广泛的应用于各个领域的生物学研究中。文章以454测序系统为例, 综述了新一代测序系统的原理、优缺点, 及其在植物转录组研究中的应用, 并对其在植物研究领域中可能的发展应用方向进行了展望。

关键词: 新一代测序平台, 454测序技术, 植物转录组, EST, SNP

Abstract: With the development of DNA sequencing techniques, the next-generation sequencing (NGS) techniques with the characteristics of high-throughput and low cost have become the first choice for more and more researchers to carry out the biological researches. Among the next-generation sequencing techniques, the 454 sequencing platform is the first com-mercially available and relatively mature one and widely used in various fields of biological research. Taking 454 sequenc-ing platform as an example, we illustrate the advantages and disadvantages of NGS technical principles, review their appli-cations in plant transcriptome, and outlook their future development and applications in plant research field.

Key words: EST, SNP, next generation sequencing techniques (NGS), 454 pyrosequencing technology, plant transcriptome