| [1] Kaledin AS, Slyusarenko AG, Gorodetski? SI. Isolation and properties of DNA polymerase from extreme thermo-phylic bacteria Thermus aquaticus YT-1. Biokhimiia, 1980, 45(4): 644-651.[2] Chien A, Edgar DB, Trela JM. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol, 1976, 127(3): 1550-1557.[3] Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol, 1987, 155: 335-350.[4] Engelke DR, Krikos A, Bruck ME, Ginsburg D. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal Biochem, 1990, 191(2): 396-400.[5] Lawyer FC, Stoffel S, Saiki RK, Chang SY, Landre PA, Abrarnson RD, Gelfand DH. High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Genome Res, 1993, 2(4): 275-287.[6] Lawyer FC, Stoffel S, Saiki RK, Myambo K, Drummond R, Gelfand DH. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J Biol Chem, 1989, 264(11): 6427-6437.[7] Abu Al-Soud W, Rådström P. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl Environ Microbiol, 1998, 64(10): 3748-3753.[8] Pavlov AR, Pavlova NV, Kozyavkin SA, Slesarev AI. Recent developments in the optimization of thermostable DNA polymerases for efficient applications. Trends Biotechnol, 2004, 22(5): 253-260.[9] Rittié L, Perbal B. Enzymes used in molecular biology: a useful guide. J Cell Commun Signal, 2008, 2(1-2): 25-45.[10] Grimm E, Arbuthnot P. Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures. Nucleic Acids Res, 1995, 23(21): 4518-519.[11] Pluthero FG. Rapid purification of high-activity Taq DNA polymerase. Nucleic Acids Res, 1993, 21(20): 4850-4851.[12] Yang ZG, Ding YM, Zhang YH, Liu FH. Rapid purification of truncated Taq DNA polymerase Stoffel fragment by boiling lysis of bacterial expression cultures. Biotechnol Appl Biochem, 2008, 50(2): 71-75.[13] Gräslund T, Nilsson J, Lindberg AM, Uhlén M, Nygren PÅ. Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein. Protein Expr Purif, 1997, 9(1): 125-132.[14] Melissis S, Labrou NE, Clonis YD. One-step purification of Taq DNA polymerase using nucleotide-mimetic affinity chromatography. Biotechnol J, 2007, 2(1): 121-132.[15] Brandis JW, Johnson KA. High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli. Protein Expr Purif, 2009, 63(2): 120-127.[16] Kim YJ, Lee HS, Bae SS, Jeon JH, Lim JK, Cho Y, Nam KH, Kang SG, Kim SJ, Kwon ST, Lee JH. Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp. NA1. J Microbiol Biotechnol, 2007, 17(7): 1090-1097.[17] Moreno R, Haro A, Castellanos A, Berenguer J. High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus. Appl Environ Microbiol, 2005, 71(1): 591-593.[18] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72(1-2): 248-254.[19] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227(5259): 680-685.[20] Roa |
