摘要:
提出了一种新的DNA芯片的封闭方法:1-碘丙烷封闭方法,并对1-碘丙烷和琥珀酸酐两种不同的封闭方法的封闭效果进行了比较实验,研究封闭试剂、封闭时间对氨基DNA芯片杂交结果所产生的影响。结果表明: 1-碘丙烷封闭效果明显好于琥珀酸酐的封闭效果,用1-碘丙烷封闭后的芯片进行杂交,芯片杂交信号强、背景噪声低,灵敏度高,整个封闭过程实验操作简单,时间短,1h即可达到所需效果。1-碘丙烷可以作为一种很好的DNA芯片封闭试剂。Abstract: Suggested a new blocking method for DNA microarray: 1- iodo -propane .we compared the difference between the two block reagents and the blocking results in different blocking time. The results showed that 1-I-prppane is batter-blocking reagent than succinic anhydride in DNA chip.1h blocking time is enough for blocking.