遗传 ›› 2000, Vol. 22 ›› Issue (3): 167-168.

• 论文 • 上一篇    下一篇

一种新的DNA银染方法

方卫国;韦宇拓;裴炎 FANG Wei-guo;WEI Yu-tuo;PEI Yan   

  1. 西南农业大学生物技术中心, 重庆 北碚 400716 Biotechnology Research Center,Southwest Agricultural University,Chongqing 400716,China
  • 收稿日期:1900-01-01 出版日期:2000-06-10 发布日期:2000-06-10

An Effective Silver Staining Protocol for DNA

  • Received:1900-01-01 Online:2000-06-10 Published:2000-06-10

摘要: 本文介绍一种有效的聚丙烯酰胺中的DNA银染方法,其具有背景浅、快速、灵敏度高等特点。利用该方法对MarkerX和棉花的RAPD产物进行银染,取得了较满意的结果。说明该方法适于聚丙烯酰胺中的DNA银染。
Abstract:An effective silver staining protocol for DNA in PAGE was introduced in this article,it characterized weak background,sensitivity,time saving .Based on this protocol,MarkerX and products of RAPD were stained,the satisfactory staining map indicated that this silver staining protocol was applicable to DNA in PAGE.

关键词: MarkerX, silver staining, RAPD产物 ey words, 聚丙烯酰胺, 银染