遗传 ›› 1996, Vol. 18 ›› Issue (1): 1-3.

• 论文 •    下一篇

SRY盒基因在斑马鱼和胡子鲇中的保守性分析

周荣家; 余其兴; 程汉华 ZHOU Rong-Jia;YU Qi-Xing;CHENG Han-Hua   

  1. 武汉大学生命科学学院, 武汉 430072 College of Life Sciences,Wuhan University,Wuhan 430072
  • 收稿日期:1900-01-01 出版日期:1996-02-10 发布日期:1996-02-10

The Conservation Analysis of SRY-box Genes in Clarias fuscus and Brachydanio rerio

  • Received:1900-01-01 Online:1996-02-10 Published:1996-02-10

摘要: 本文采用人类SRY基因探针,与EcoRI消化的中国的胡子鲇(Clarias fusc us)和美国的斑马鱼(Brachydanio rerio)基因组DNA进行了Southern印迹杂交分析。首次在这两种鱼中发现了SRY盒基因及其存在特征;在斑马鱼中发现了明显的限制性片段长度多态性; 并深入分析了SRY盒基因的进化保守性。
Abstract:Using,Southern bloting analysis,EcoRI-digested genomic DNA from Clarias fuscus and Brachydanio rerio were hybridizae to human SRY probe.Hybridization occurred in both male and female of the two species.RFLP associated with SRY-box genes was observed in Brachydanio rerio.The conservation of SRY-box genes was analysed.

关键词: SRY盒, 鱼类, RFLP
Key words,
SRY-box, Fishes, vWF基因, 单链构型多态性, 可变数量串联重复

Key words: SSCP (single-strand comformation polymorphism), vWF gene VNTR (variable number of tandem repeats)