虾虎鱼类线粒体全基因组序列结构特征分析及系统发育关系探讨

doi:10.3724/SP.J.1005.2013.01391

遗传 ›› 2013, Vol. 35 ›› Issue (12): 1391-1402.doi: 10.3724/SP.J.1005.2013.01391

虾虎鱼类线粒体全基因组序列结构特征分析及系统发育关系探讨

金逍逍, 孙悦娜, 王日昕, 汤达, 赵盛龙, 徐田军

浙江海洋学院海洋科学与技术学院, 鱼类分子遗传与免疫进化实验室, 舟山 316000

收稿日期:2013-05-22 修回日期:2013-08-07 出版日期:2013-12-20 发布日期:2013-11-25
通讯作者: 徐田军, 副教授, 研究方向：鱼类分子免疫及分子进化。 E-mail:tianjunxu@163.com
作者简介:金逍逍, 硕士, 专业方向：鱼类分子系统学。E-mail: jinxiaoxiaoyy@163.com
基金资助:
国家自然科学基金项目(编号：31272661)和浙江省自然科学基金项目(编号：LY13C040001)资助

Characteristics and phylogenetic analysis of mitochondrial genome in the gobies

JIN Xiao-Xiao, SUN Yue-Na, WANG Ri-Xin, TANG Da, ZHAO Sheng-Long, XU Tian-Jun

Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China

Received:2013-05-22 Revised:2013-08-07 Online:2013-12-20 Published:2013-11-25
Contact: tianjun Xu E-mail:tianjunxu@163.com

摘要/Abstract

摘要：

虾虎鱼类体态变异大、体型小、种类多, 形态鉴定及谱系分类较为困难。为深入开展虾虎鱼类的鉴定、分类及遗传进化等研究, 文章对已获得的26种虾虎鱼线粒体全基因组进行分析。结果发现, 虾虎鱼类线粒体基因组的基因组成及排列模式与大多数脊椎动物线粒体基因组特征基本一致; 由于不同物种的控制区存在不同数量的重复序列而导致基因组序列长度存在明显的差异; 26种虾虎鱼线粒体全基因组序列及不同基因中A+T的含量均超过50%, 并存在碱基G偏倚现象。基于37个编码基因序列, 利用Kimura双参数法计算遗传距离, 发现矛尾刺虾虎鱼与斑尾刺虾虎鱼、斑纹舌虾虎鱼与钝吻舌虾虎鱼分别为同种异名。通过对26种虾虎鱼线粒体基因组控制区序列的比较, 识别了终止结合序列区、中央保守区及保守序列区。利用26种虾虎鱼线粒体基因组的36个编码基因序列构建系统发育树, 发现部分聚类结果不同于传统的形态学分类方式, 虾虎鱼科中的5个亚科出现了明显的分化, 近盲虾虎鱼亚科、背眼虾虎鱼亚科、瓢虾虎鱼亚科亲缘关系较近而聚成一大支, 然后与拟虾虎鱼亚科种类形成姐妹类群, 虾虎鱼亚科与其它的4个亚科亲缘关系较远, 单独成为一个类群。根据分子钟估算结果推测虾虎鱼科物种可能起源于始新世晚期至渐新世时段, 在中新世进一步分化为具有现代表征的虾虎鱼种类。

关键词: 虾虎鱼类, 线粒体基因组, 结构特征, 系统发育基因组学

Abstract:

The vast number of species, small size and high variation of morphology make the morphological identification and classification of gobies very difficult. In this study, the complete mitochondrial genome (mitogenome) of 26 species of gobies was analyzed, aiming at accumulating the molecular information on the identification, classification and molecular evolution of gobies. The results showed that the gene composition and arrangement of mitogenome of gobies are similar to most vertebrates. Due to various degrees of repetitive sequences in the control region, the mitogenome of 26 gobies exhibits a great variation in length. The A+T content of the mitogenome is greater than 50% and the lowest frequency is for G among the four bases. Thirty-seven coding gene sequences were used to calculate the average Kimura 2-parameter genetic distance of 26 species of gobies. Acanthogobius hasta and A. ommaturus, Glossogobius olivaceus and G. circumspectus were synonyms, respectively. By comparing the control region sequences of 26 gobies, the terminal associated sequences, central conserved sequence block and conserved sequence block were identified, respectively. Thirty-six coding gene sequences of 26 gobies were used to construct the phylogenetic tree and the results were different from the traditional morphological classification. The five subfamilies of Gobiidae were obviously evolved: Amblyopinae, Oxudercinae and Sicydiinae were clustered into a group and then formed a sister group with Gobionellinae; the fishes of Gobiinae had distant relationship with the four subfamilies and formed a group alone. Molecular clock analysis estimated that gobies probably originated in the late Eocene to Oligocene time and further evolved into modern characteristic gobies in the Miocene.

Key words: Gobies, mitochondrial genome, structure characteristic, phylogenomic analysis

金逍逍孙悦娜王日昕汤达赵盛龙徐田军. 虾虎鱼类线粒体全基因组序列结构特征分析及系统发育关系探讨[J]. 遗传, 2013, 35(12): 1391-1402.

JIN Xiao-Xiao, SUN Yue-Na, WANG Ri-Xin, TANG Da, ZHAO Sheng-Long, XU Tian-Jun. Characteristics and phylogenetic analysis of mitochondrial genome in the gobies[J]. HEREDITAS, 2013, 35(12): 1391-1402.

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虾虎鱼类线粒体全基因组序列结构特征分析及系统发育关系探讨

Characteristics and phylogenetic analysis of mitochondrial genome in the gobies

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