遗传 ›› 1997, Vol. 19 ›› Issue (1): 19-22.

• 论文 • 上一篇    下一篇

节节麦×大麦杂种胚再生植株的细胞遗传学研究

李锁平; 胡玉欣; 刘孝峰; 杜汪蕾 LI Suo-Ping;HU Yu-Xin;LIU Xiao-Feng;DU Wang-Lei   

  1. 河南大学生物学系, 开封 475001 Department of Biology,Henan University,Kaifeng 475001
  • 收稿日期:1900-01-01 出版日期:1997-02-10 发布日期:1997-02-10

Cytogenetics of Regenerated Plants from Embryo Callus of the Hybrids betwen Aegilops tauschii and Hordeum vulgare

  • Received:1900-01-01 Online:1997-02-10 Published:1997-02-10

摘要: 以节节麦(2n=14)为母本和大麦(2n=14)进行杂交,两组合平均结实率为31 .11%。对31个幼胚进行愈伤组织诱导培养,其中2个形成全能性愈伤组织进而分化出再生杂种植株。细胞学观察表明,杂种胚再生植株均是染色体加倍的节节麦-大麦双二倍体(2n=28)或染色体再加倍的多倍体。节节麦-大麦双二倍体在减数分裂中期Ⅰ染色体配对平均构型为14.79Ⅰ+1.95Ⅱ+ 4.53Ⅱ′+0.01Ⅲ,表现为自交不育。杂种胚再生植株染色体数目再加倍的多倍体有少数产生了自交种子。
Abstract:Hybridizations between Aegilops tauschii(2n=14)and Hordeum vulgare(2n=14)were made using Ae.tauschii as female.The mean frequency of seed set was 31.11% in two cross combinations,13 hybrid embryos were cultured for inducing callus,of which 2 produced totipotent callus,and plants were regenerated from them.These plants were Ae.tauschii-H.vulgare amphidiploids with doubling chromosome number(2n=28)and octoploids with repeatedly doubling chromosome number(2n=50~56).The Ae.tauschii-H.vulgare amphidiploids were sterile and their average chromosome pairing at MI were 14.79I+1.95II+4.53II′+0.01III.Some octoploid(2n=56)plants produced seeds after selfing.

关键词: 植株再生, 染色体加倍, 基因不育
Key words,
大麦, 节节麦, 基因分的, 新基因, 籼稻, 稻瘟病

Key words: indica rice, Gene analysis, New gene, Rice blast disease