遗传 ›› 2001, Vol. 23 ›› Issue (4): 333-786.

• 论文 • 上一篇    下一篇

用超常的复性温度改善小麦RA PD分析的效果

刘红彦;牛永春;钟鸣 LIU Hong-yan;NIU Yong-chun;ZHONG Ming   

  1. 中国农业科学院植物保护研究所,北京 100094 Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China
  • 收稿日期:1900-01-01 出版日期:2001-08-10 发布日期:2001-08-10

Better RAPD Patterns Obtained by Using High Annealing Temperatures in Wheat

  • Received:1900-01-01 Online:2001-08-10 Published:2001-08-10

摘要: 用38~66℃不同复性温度处理,比较了18条随机引物的扩增结果.发现复性温度在40~50℃之间均有数量不等的扩增产物,不同引物的最高复性温度不同,有些引物用60℃以上的复性温度仍有扩增产物.一些在35~38℃的复性温度下非特异性产物较多的引物,通过大幅度提高复性温度,能提高扩增产物的特异性,获得清晰的RAPD带型。
Abstract:To decrease nonspecific products and obtain clear RAPD patterns,18 10- mer random primers were tested at different annealing temperatures.The results indicated that all the amplification can be performed when the annealing temperature is in the range of 40~50℃.There were a few primers with which the amplification was still performed when the annealing temperature is above 60℃.By using high annealing temperatures,some primers which produce more nonspecific product at the annealing temperatures of 35~38℃ could generate reproducible and distinct bands.

关键词: RAPD, 特异性
Key words,
复性温度, annealing temperature, RAPD