遗传 ›› 2001, Vol. 23 ›› Issue (4): 336-791.

• 论文 • 上一篇    下一篇

野生型发根农杆菌K599的解毒

向太和1;杨剑波1;David A.Somers2 XIANG Tai-heI;YANG Jian-bo1;David A.Somers2   

  1. 1.安徽省农业科学院水稻研究所、农业部水稻遗传育种重点开放实验室合肥230031 2.Department of Agronomy and Plant Genetics,University of Minnesota,St.Paul,MN 55108,USA 1.Rice Research Institute,Key Laboratory of Rice Genetics and Breeding of Agricultural Ministry,A nhui Academy of Agricultural Scienecs,Hefei 230031,China;
  • 收稿日期:1900-01-01 出版日期:2001-08-10 发布日期:2001-08-10

Disarming of Wild Type Agrobacterium rhyzogenes K599

  • Received:1900-01-01 Online:2001-08-10 Published:2001-08-10

摘要: 本研究利用DNA重组技术构建了来源于质粒pJBJ106、pBluescriptSK(+)和pUCD800的新质粒pXT3sacB,利用该质粒通过同源重组切除了野生型发根农杆菌K599中Ri质粒的T-DNA.解毒后的K599获得了氨苄/羧苄青霉素抗性和10%蔗糖抑制生长的选择标记.解毒后的K599菌株可能对农杆菌转基因技术是有益的。
Abstract:In this study,the new plasmid pXT3 sacB was constructed from pJBJ106,pBluescript SK( + ) and pUCD800.The wild type T-DNA of Agrobacterium rhyzogenes K599 which induces hair root was deleted with pXT3sacB by homologous recombinant.A disarming K599 contains selective markers of resistance to ampicillin/carbeneillin and the growth inhibitation by 10% sucrose.It could be a novel useful strain for gene transfer via Agrobacterium- mediated method.

关键词: 发根农杆菌K599, Agrobacterium rhyzogenes K599, 解毒
Key words,
disarming