遗传 ›› 2001, Vol. 23 ›› Issue (4): 336-791.
向太和1;杨剑波1;David A.Somers2 XIANG Tai-heI;YANG Jian-bo1;David A.Somers2
摘要: 本研究利用DNA重组技术构建了来源于质粒pJBJ106、pBluescriptSK(+)和pUCD800的新质粒pXT3sacB,利用该质粒通过同源重组切除了野生型发根农杆菌K599中Ri质粒的T-DNA.解毒后的K599获得了氨苄/羧苄青霉素抗性和10%蔗糖抑制生长的选择标记.解毒后的K599菌株可能对农杆菌转基因技术是有益的。
Abstract:In this study,the new plasmid pXT3 sacB was constructed from pJBJ106,pBluescript SK( + ) and pUCD800.The wild type T-DNA of Agrobacterium rhyzogenes K599 which induces hair root was deleted with pXT3sacB by homologous recombinant.A disarming K599 contains selective markers of resistance to ampicillin/carbeneillin and the growth inhibitation by 10% sucrose.It could be a novel useful strain for gene transfer via Agrobacterium- mediated method.