遗传 ›› 2002, Vol. 24 ›› Issue (3): 293-296.

• 论文 • 上一篇    下一篇

饲料原料中转基因成分的PCR检测

王颖1;余道坚1;康林1;章桂明1;金显忠1;杨伟东1;黄佩卿1;吴琼1;陈枝楠1;储成才2;程颖慧3 WANG Ying1;YU Dao-jian1;KANG Lin1;ZHANG Gui-ming1;JIN Xian-zhong1;YANG Wei-dong1;HUANG Pei-qing1;WU Qiong1;CHEN Zhi-nan1;CHU Cheng-cai2;CHENG Ying-hui3   

  1. 1.深圳出入境检验检疫局,深圳 518010; 2.中国科学院遗传与发育生物学研究所,北京100101; 3.华南农业大学,广州 510642 1.Shenzhen Exit-Entry Inspection and Quarantine Bureau,Shenzhen 518100 China; 2.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China; 3.South China Agricultural University,Guangzhou 510642 China
  • 收稿日期:1900-01-01 出版日期:2002-06-10 发布日期:2002-06-10

PCR Detection of Transgenic Elements in Feed Raw Material

  • Received:1900-01-01 Online:2002-06-10 Published:2002-06-10

摘要: 采用PCR检测方法从饲料的主要原料豆粕、玉米蛋白粉中成功地检出启动子35S (35S-promoter,originated from cauliflower mosaic virus)、终止子NOS (nopaline synthase-terminator,derived from Agrobacterium tumefaciens)、耐除草剂基因EPSPS(5-enolpyruvylshikimate-3-phosphate synthase)和抗虫基因CryIA(b)(delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)等转基因成分,并通过扩增玉米自身蛋白基因Zein( a protein extracted from corn gluten)及大豆自身基因Lectin(chitin-binding protein)的引物和阴阳性对照、阴阳性质控,避免假阳性、假阴性结果。该方法已在口岸进口饲料原料转基因检测中得到初步应用。
Abstract:Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus),NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS(5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b)(delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal,respectively.Endogenous corn Zein(a protein extracted from corn gluten) gene,soybean Lectin(chitin-binding protein) gene and negative,positive control were applied for avoiding false results.The method established here has been succeessfully applied in detecting transgenic elements in imported feed raw material.

关键词: 饲料, 豆粕, 玉米蛋白粉
Key words,
转基因, PCR