摘要: 以美洲黑杨(Populus deltoides Bartr.cv.“Lux”(I-69/55))为母本,欧美杨(P.euramericana cv.I-45)为父本得到的F1为材料,其中I-69对黑斑病表现为高度抗病,I-45为高度感病。利用分离群体混合分析(bulked segregrants analysis,BSA)技术建立两个DNA池(感病池和抗病池),筛选出了与抗黑斑病性状基因相连锁的RAPD标记:OPAI17―1550、OPAI13―900。利用选择性基因型分析法进行标记―抗黑斑病基因连锁分析,两标记与抗黑斑病基因遗传距离分别为29.9cM和37.4cM。
Identification of Markers Linked to Resistance Locus of Marssonina Leaf Spot in Poplars by Bulked Segregant Analysis(BSA)
ZHANG Bo1,HUANG Min-ren1,ZHUGE Qiang1,HAN Zheng-min1,YIN Tong-ming1,PAN Hui-xin1,ZHU Li-huang2,WU Rong-ling3,WANG ming-xiu1
1.The Key Laboratory of Forest Tree Genetic Engineering,Nanjing Forestry University,Nanjing,210037,China;
2.The Institute of Genetics and Developmental Biology,Chinese Academy of Science,Beijing 100101,China;
3.Department of Statistics,University of Florida,Gainesville,Florida 32611,USA
Abstract:DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA).The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv.“Lux”(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible).Out of 114 RAPD primers,four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus.The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.
Key words:BSA; RAPD; selective genotype analysis; Marssonina leaf spot