遗传 ›› 2003, Vol. 25 ›› Issue (1): 65-68.

• 论文 • 上一篇    下一篇

影响多重PCR扩增效果的因素

黄银花1;胡晓湘1;徐慰倬1;高宇1;冯继东1;孙汉2;李宁1   

  1. 1.中国农业大学农业生物技术国家重点实验室,北京 100094;2.江西农业大学动物科技学院,南昌 330045
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-02-10 发布日期:2003-02-10

The Factors Affecting the Efficiency of Mutiplex PCR

HUANG Yin-Hua1;HU Xiao-Xiang1;XU Wei-Zhuo1;GAO Yu1;FENG Ji-Dong1;SUN Han2;LI Ning1   

  1. 1.National Laboratories for Agrobiotechnology,China Agricultural University,Beijing 100094,China;
    2.College of Animal Science,Jiangxi Agricultural University,Nanchang 330045,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-02-10 Published:2003-02-10

摘要: 不同循环参数、PCR缓冲液及反应体积的对比实验表明,循环参数中退火温度和时间、延伸时间及PCR缓冲液的成分影响多重PCR的扩增效果,而反应体积、循环次数对其扩增效果影响较小。

关键词: 反应体积, 多重PCR, PCR缓冲液, 循环参数

Abstract: In the research,the outputs of different cycle parameters,PCR buffer and reaction volumes are compared.The results indicated that the annealing temperature,annealing time,elongated time and ingredient of PCR buffer affected mutiplex PCR,but the reaction volume and cycle number had few effect on it.