遗传 ›› 2003, Vol. 25 ›› Issue (3): 299-301.

• 论文 • 上一篇    下一篇

小麂Sry基因的克隆和测序

鲁晓瑄;张 悦;单祥年 LU Xiao-Xuan;ZHANG Yue;SHAN Xiang-Nian   

  1. 东南大学医学院遗传学研究中心,南京 210009 Genetic Research Center,Medical College of Southeast University,Nanjing 210009,China
  • 收稿日期:1900-01-01 出版日期:2003-06-10 发布日期:2003-06-10

Cloning and Sequencing of Sry Gene of Muntiacus

  • Received:1900-01-01 Online:2003-06-10 Published:2003-06-10

摘要: 应用人的性别决定基因SRY(Sex-determining Region Y gene,SRY)中HMG框内的一对引物,对小麂细胞株的基因组DNA进行PCR扩增,得到雄性小麂细胞的220bp扩增产物,而在雌性小麂细胞中未发现扩增产物。将雄性小麂细胞的220bp扩增产物通过T-A互补法克隆到质粒pGEM-T 载体中,筛选阳性克隆进行DNA测序。测序结果表明小麂Sry基因保守序列与人的SRY基因保守区相同碱基的比值为152/184,达到82.6%。提示小麂Sry基因与人的SRY基因存在着较高的同源性,说明SRY基因在进化过程中高度保守。
Abstract:Using the primers from SRY gene——HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains,a 220bp fragment was obtained in the male but not in the female.The 220bp fragment was cloned into the pGEM-T vector using T/A clone method.The identified positive clone was sequenced.The result shows that 82.6% nucleotides(152bp/184bp) are homologous between Muntiacus Sry and human SRY gene.It suggests that SRY is highly conserved during evolution.

关键词: SRY基因
Key words,
sex-determined, Muntiacus, 性别决定, 小麂