用模式生物青鳉概观硬骨鱼性别决定及性分化研究进展

doi:10.16288/j.yczz.17-140

遗传 ›› 2017, Vol. 39 ›› Issue (6): 441-454.doi: 10.16288/j.yczz.17-140

• 特邀综述 • 下一篇

用模式生物青鳉概观硬骨鱼性别决定及性分化研究进展

亢逸¹(),关桂君¹(),洪云汉²

1. 上海海洋大学水产与生命学院,鱼类性控及干细胞实验室,上海 201306
2. 新加坡国立大学,生物系遗传发育研究室,新加坡 119260

收稿日期:2017-04-17 修回日期:2017-05-15 出版日期:2017-06-20 发布日期:2017-05-26
作者简介:亢逸,硕士研究生,研究方向：水产养殖学。E-mail: 510327775@qq.com|关桂君，博士，特聘教授，研究方向：鱼类生殖生物学。E-mail: gjguan@shou.edu.cn|关桂君教授课题组主要致力于鱼类性别分化和性别调控的分子机制研究。其本人在日本国立基础生物学研究所攻读博士期间,在PNAS上发表了有关虹鳟卵细胞成熟诱导关键因子20β-羟基类固醇脱氢酶分子克隆和活性基团定位分析的论文。博士后期间分子克隆并发现了罗非鱼雌雄性差表达的相关基因dmrt1和dmo,首先揭示了dmrt基因在鱼类性腺分化发育中的重要作用。近年来同新加坡国立大学遗传发育研究室洪云汉教授(世界首创半克隆动物Holly青鳉和脊椎动物首株单倍体胚胎干细胞系的建立)团队一起,利用锌指核糖核酸酶,直接基因敲除和转基因过表达青鳉性腺体细胞衍生因子gsdf,功能分析并证明了gsdf在精巢和卵巢分化发育过程中的重要作用,部分结果发表在了DNA Research、Marine Biotechnol、Sci Reports、Mech Dev等国际主流期刊上。
基金资助:
上海市自然科学基金(16ZR1415200)

Insights of sex determination and differentiation from medaka as a teleost model

Yi Kang¹(),Guijun Guan¹(),Yunhan Hong²

1. Laboratory of Fish Sex Differentiation and Stem Cell Biology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
2. Department of Biological Sciences, National University of Singapore, Singapore 119260, Singapore

Received:2017-04-17 Revised:2017-05-15 Online:2017-06-20 Published:2017-05-26
Supported by:
the Natural Science Foundation of Shanghai(16ZR1415200)

摘要/Abstract

摘要：

鱼类性别决定和性分化呈多元性,既有雌雄同体也有雌雄异体。性腺的雌雄性分化过程受遗传和环境因素(如温度、光照、激素和pH值等)影响,具有可逆可塑性。随着生物技术和基因组学的迅速发展,近年来脊椎动物性别决定和性分化的研究有了重大进展和显著突破。本文通过聚焦青鳉及其他硬骨鱼纲保守存在的dmrt1、gsdf、amh等雄性因子,探讨硬骨鱼普遍存在的雌雄性别可塑可逆信号通路,并介绍了新的基因组编辑和性控育种技术,为单性选育等水产养殖技术的研发提供参考。

关键词: 青鳉, 性别决定, 性分化

Abstract:

The mechanisms of sex determination and differentiation in fish are highly divergent with a broad range of gonadal differentiation types from hermaphroditism to gonochorism. Multiple triggers regulate the process of sexual differentiation including genetic or environmental factors (temperature, light, hormones and/or pH value, etc.). In recent years, with the advances of molecular technologies and genetic engineering approaches, there are significant breakthroughs in identifying the master genes of vertebrate sex determination and differentiation. In this review, we explore the fundamental and molecular mechanisms underlying the sexual differentiation in teleost fish, using medaka (Oryzias latipes) as a model. We focus on the male pathways and factors, particularly on dmrt1, gsdf and amh genes involved in testicular differentiation, sexual reversal and plasticity. It is anticipated that new techniques will likely be developed in the field of sex manipulations and monosex breeding for fish aquaculture in the future.

Key words: medaka, sex determination, sexual differentiation

亢逸,关桂君,洪云汉. 用模式生物青鳉概观硬骨鱼性别决定及性分化研究进展[J]. 遗传, 2017, 39(6): 441-454.

Yi Kang,Guijun Guan,Yunhan Hong. Insights of sex determination and differentiation from medaka as a teleost model[J]. Hereditas(Beijing), 2017, 39(6): 441-454.

参考文献

[1]	Devlin RH, Nagahama Y. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences . Aquaculture, 2002, 208(3-4): 191-364.
[2]	Volff JN, Nanda I, Schmid M, Schartl M. Governing sex determination in fish: regulatory putsches and ephemeral dictators. Sex Dev, 2007, 1(2): 85-99.
[3]	Stelkens RB, Wedekind C. Environmental sex reversal, Trojan sex genes, and sex ratio adjustment: conditions and population consequences. Mol Ecol, 2010, 19(4): 627-646.
[4]	Nanda I, Kondo M, Hornung U, Asakawa S, Winkler C, Shimizu A, Shan ZH, Haaf T, Shimizu N, Shima A, Schmid M, Schartl M. A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes. Proc Natl Acad Sci USA, 2002, 99(18): 11778-11783.
[5]	Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T, Morrey CE, Shibata N, Asakawa S, Shimizu N, Hori H, Hamaguchi S, Sakaizumi M. DMY is a Y-specific dm-domain gene required for male development in the medaka fish. Nature, 2002, 417(6888): 559-563.
[6]	Aida T. On the inheritance of color in a fresh-water fish, Aplocheilus latipes temmick and schlegel, with special reference to sex-linked inheritance. Genetics, 1922, 7(1): 115.
[7]	Yamamoto TO. Artificially induced sex-reversal in genotypic males of the medaka (Oryzias latipes). J Exp Zool, 1953, 123(3): 571-594.
[8]	Zhang X, Guan GJ, Li MY, Zhu F, Liu QZ, Naruse K, Herpin A, Nagahama Y, Li JL, Hong YH. Autosomal gsdf acts as a male sex initiator in the fish medaka. Sci Rep, 2016, 6: 19738.
[9]	Guan GJ, Sun KQ, Zhang X, Zhao XM, Li MY, Yan Y, Wang YZ, Chen JB, Yi MS, Hong YH. Developmental tracing of oocyte development in gonadal soma-derived factor deficiency medaka (Oryzias latipes) using a transgenic approach. Mech Dev, 2017, 143: 53-61.
[10]	Sun KQ, Fan LD, Wang CY, Yang XY, Zhu SG, Zhang X, Chen XW, Hong YH, Guan GJ. Loss of gonadal soma derived factor damaging the pituitary-gonadal axis in medaka. Sci Bull, 2017, 62(3): 159-161.
[11]	Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ, Foster JW, Frischauf AM, Lovell-Badge R, Goodfellow PN. A gene from the human sex- determining region encodes a protein with homology to a conserved dna-binding motif. Nature, 1990, 346(6281): 240-244.
[12]	Koopman P, Gubbay J, Vivian N, Goodfellow P, Lovell-Badge R. Male development of chromosomally female mice transgenic for Sry. Nature, 1991, 351(6322): 117-121.
[13]	Raymond CS, Shamu CE, Shen MM, Seifert KJ, Hirsch B, Hodgkin J, Zarkower D. Evidence for evolutionary conservation of sex-deter

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用模式生物青鳉概观硬骨鱼性别决定及性分化研究进展

Insights of sex determination and differentiation from medaka as a teleost model

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