转录组测序揭示细胞周期通路参与鸡腹脂沉积

doi:10.16288/j.yczz.19-098

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Hereditas(Beijing) ›› 2019, Vol. 41 ›› Issue (10): 962-973.doi: 10.16288/j.yczz.19-098

• Research Article • Previous Articles Next Articles

The cell cycle pathway regulates chicken abdominal fat deposition as revealed by transcriptome sequencing

Jiahui Chen,Xueyi Ren,min Li,Shiyi Lu,Tian Cheng,Liangtian Tan,Shaodong Liang,Danlin He,Qingbin Luo,Qinghua Nie,Xiquan Zhang,Wen Luo()

Guangdong Key Laboratory of Agricultural Animal Genomics and Molecular Breeding, Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received:2019-04-09 Revised:2019-07-05 Online:2019-10-20 Published:2019-08-26
Contact: Luo Wen E-mail:luowen729@scau.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China No.(31702105);The China Agriculture Research System No.(CARS-41-G03);The Science and Technology Program of Guangzhou, China No.(201804020088)

Abstract

Abstract:

With the improvement of growth traits and feed conversion rate, the abdominal fat rate of Chinese local breeds of broilers has been increasing. Excessive abdominal fat deposition not only reduces the slaughter rate and disease resistance of broiler chickens, but also produces waste due to the difficulty of fat treatment. In order to study the regulatory genes and pathways involved in abdominal fat deposition of broilers, we used high-fat diets to feed the Xinghua Chicken, which is a Chinese local breed. Two weeks after feeding, we found that the abdominal fat weight and rate of broilers in the high-fat diet group increased significantly, and the diameter and area of abdominal fat cells also increased significantly. Transcriptome sequencing of abdominal fat and livers showed that the differentially expressed genes in the abdominal fat were mainly enriched in the cell cycle, peroxisome proliferator- activated receptor (PPAR) and extracellular matrix (ECM) receptor signaling pathways. The differentially expressed genes in livers were also significantly enriched in the cell cycle pathway, as well as in the steroid biosynthesis and PPAR signaling pathway. By analyzing the common differentially expressed genes in abdominal fat and liver tissues, we found that these genes were also enriched in cell cycle. Finally, we used the chicken LMH (chicken hepatoma cell) cell line and chicken ICP (immortalized chicken preadipocytes) cell line to do the in vitro validation assays. We used high-fat and common medium to culture the cells. The results showed that after 48 hours, the high-fat medium could significantly promote cell cycle and increase the number of cells in S phase. Additionally, qRT-PCR results showed that the high-fat medium could significantly promote the expression of genes related to cell cycle. In conclusion, we found that high-fat diets activate the cell cycle progression of chicken hepatocytes and preadipocytes, promote cell proliferation, and then increase abdominal fat deposition.

Key words: Xinghua chicken, high-fat diet, abdominal fat deposition, cell cycle, differentially expressed genes

Jiahui Chen, Xueyi Ren, min Li, Shiyi Lu, Tian Cheng, Liangtian Tan, Shaodong Liang, Danlin He, Qingbin Luo, Qinghua Nie, Xiquan Zhang, Wen Luo. The cell cycle pathway regulates chicken abdominal fat deposition as revealed by transcriptome sequencing[J]. Hereditas(Beijing), 2019, 41(10): 962-973.

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