基于油桐种子3个不同发育时期转录组的油脂合成代谢途径分析

doi:10.3724/SP.J.1005.2013.01403

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (12): 1403-1414.doi: 10.3724/SP.J.1005.2013.01403

Analysis of oil synthesis metabolism pathways based on transcrip-tomechanges in tung oil tree’s seeds during three different develop-ment stages

CHEN Hao^1,2, JIANG Gui-Xiong^1,2, LONG Hong-Xu^1,2, TAN Xiao-Feng^1,2

1. The Key Lab of Cultivation and Protection for Non-wood Forest Trees of Education Ministry, Central South University of Forestry and Technology, Changsha 410004, China;
2. The Key Lab of Non-wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, China

Received:2013-06-27 Revised:2013-09-07 Online:2013-12-20 Published:2013-11-25

Abstract

Abstract:

Tung oil tree (Vernicia fordii) is one of the important woody oil plants in China. Past researches on tung oil tree mainly focu on the cultivation and conventional breeding while the molecular mechanisms related to tung oil synthesis are still uncovered. We compared transcriptome of tung oil tree’s seeds at three different oil synthesis stages using RNA-seq technology and then obtained a lot of differentially expressed Unigenes. Through GO classification and pathway enrichment analysis, all of these differentially expressed Unigenes were classified into 128 metabolism pathways including fatty acid biosynthesis and glycerophospholipid metabolism which are involved in oil synthesis. Some homologous proteins of key enzymes were obtained when the sequences of the Unigenes within these two pathways were aligned against KEGG data-base. Through analysis of expression profiles of these key enzyme genes during seed’s oil synthesis stage, this research not only shed light on elucidation of plant oil synthesis but also provides candidate genes for genetic improvement of tung oil tree thereby increasing the yield per unit area of tung oil tree.

Key words: Tung oil tree, oil synthesis pathway, RNA-seq, transcriptome, differentially expressed genes

CHEN Hao, JIANG Gui-Xiong, LONG Hong-Xu, TAN Xiao-Feng. Analysis of oil synthesis metabolism pathways based on transcrip-tomechanges in tung oil tree’s seeds during three different develop-ment stages[J]. HEREDITAS, 2013, 35(12): 1403-1414.

References

[1] 谭晓风, 蒋桂雄, 谭方友, 周伟国, 吕平会, 罗克明, 孙汉洲, 王承南, 马锦林, 何佳林, 梁文汇, 黄艳. 我国油桐产业化发展战略调查研究报告. 经济林研究, 2011, 29(3): 1–7. <\p>

[2] 刘金龙, 郑小江, 郑威, 田国政, 杨驰. 油桐品种五爪桐含油量及桐油质量研究. 湖北农业科学, 2011, 50(10): 2031–2035. <\p>

[3] Rock CO, Cronan JE. Escherichia coli as a model for the regulation of dissociable (typeⅡ) fatty acid biosynthesis. Biochim Biophys Acta, 1996, 1302(1): 1–16. <\p>

[4] Ohlrogge J, Browse J. Lipid biosynthesis. Plant Cell, 1995, 7(7): 957–970. <\p>

[5] Shanklin J, Cahoon EB. Desaturation and related modifi-cations of fatty acids. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 611–641. <\p>

[6] Nikolau BJ, Ohlrogge JB, Wurtele ES. Plant biotin-containing carboxylases. Arch Biochem Biophys, 2003, 414(2): 211–222. <\p>

[7] Konishi T, Shinohara K, Yamada K, Sasaki Y. Acetyl-CoA carboxylase in higher plants: most plants other than gramineae have both the prokaryotic and the eukaryotic forms of this enzyme. Plant Cell Physiol, 1996, 37(2): 117–122. <\p>

[8] Ruch FE, Vagelos PR. The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier pro-tein transacylase. J Biol Chem, 1973, 248(23): 8086–8094. <\p>

[9] White SW, Zheng J, Zhang YM, Rock CO. The structural biology of type Ⅱ fatty acid biosynthesis. Annu Rev Bio-chem, 2005, 74(1): 791–831. <\p>

[10] Fisher M, Kroon JTM, Martindale W, Stuitje AR, Slabas AR, Rafferty JB. The X-ray structure of Brassica napus β-keto acyl carrier protein reductase and its implications for substrate binding and catalysis. Structure, 2000, 8(4): 339–347. <\p>

[11] Kimber MS, Martin F, Lu Y, Houston S, Vedadi M, Dharamsi A, Fiebig KM, Schmid M, Rock CO. The struc-ture of (3R)-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Pseudomonas aeruginosa. J Biol Chem, 2004, 279(50): 52593–52602. <\p>

[12] Heath RJ, Rock CO. Enoyl-acyl carrier protein reductase (fabI) plays a determinant role in completing cycles of fatty acid elongation in Escherichia coli. J Biol Chem, 1995, 270(44): 26538–26542. <\p>

[13] Voelker T. Plant acyl-ACP thioesterases: chain-length de-termining enzymes in plant fatty acid biosynthesis. Genet Eng, 1996, 18: 111–133. <\p>

[14] Yuan L, Voelker TA, Hawkins DJ. Modification of the substrate specificity of an acyl-acyl carrier protein thio-esterase by protein engineering. Proc Natl Acad Sci USA, 1995, 92(23): 10639–10643. <\p>

[15] 周奕华, 陈正华. 植物种子中脂肪酸代谢途径的遗传调控与基因工程. 植物学通报, 1998, 15(5): 16–23. <\p>

[16] Jako C, Kumar A, Wei YD, Zou JT, Barton DL, Giblin EM, Covello PS, Taylor DC. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyl-transferase enhances seed oil content and seed weight. Plant Physiol, 2001, 126(2): 861–874. <\p>

[17] Shockey JM, Gidda SK, Chapital DC, Kuan JC, Dhanoa PK, Bland JM, Rothstein SJ, Mullen RT, Dyer JM. Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell, 2006, 18(9): 2294–2313. <\p>

[18] 汪阳东, 李元, 李鹏. 油桐桐酸合成酶基因克隆和植物表达载体构建. 浙江林业科技, 2007, 27(2): 1–5. <\p>

[19] 李建安, 孙颖, 陈鸿鹏, 刘丽娜, 郭文丹. 油桐LEAFY同源基因片段的克隆与分析. 中南林业科技大学学报, 2008, 28(4): 21–26. <\p>

[20] 李元, 汪阳东, 李鹏, 魏建民. 油桐种子FADX基因的克隆和序列分析. 安徽农业科学, 2008, 36(11): 4753–4755. <\p>

[21] 龙洪旭, 谭晓风, 张琳, 谢禄山, 陈洪. 油桐亲环素基因全长cDNA的克隆及序列分析. 中南林业科技大学学报, 2011, 31(12): 111–117. <\p>

[22] Alagna F, D'Agostino N, Torchia L, Servili M, Rao R, Pietrella M, Giuliano G, Chiusano ML, Baldoni L, Perrotta G. Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development. BMC Ge-nomics, 2009, 10(1): 399. <\p>

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Analysis of oil synthesis metabolism pathways based on transcrip-tomechanges in tung oil tree’s seeds during three different develop-ment stages

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