融入生物信息学分析的综合性探究型表观遗传学实验设计与探索

doi:10.16288/j.yczz.24-217

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Hereditas(Beijing) ›› 2025, Vol. 47 ›› Issue (5): 600-608.doi: 10.16288/j.yczz.24-217

• Genetics Teaching • Previous Articles

Design and exploration of integrating bioinformatic analysis into comprehensive and exploratory epigenetic experiments

Sainan An¹(), Huanchun Yang², Shan Jiang³, Jingxuan Li², Genfa Zhang²()

1. Platform for Experiment Teaching, Beijing Normal University at Zhuhai, Zhuhai 519087, China
2. Faculty of Arts and Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China
3. College of Education for the Future, Beijing Normal University at Zhuhai, Zhuhai 519087, China

Received:2024-07-26 Revised:2024-11-17 Online:2024-12-16 Published:2024-12-16
Contact: Genfa Zhang E-mail:ansn@bnu.edu.cn;gfzh@bnu.edu.cn
Supported by:
Teaching Construction and Reform Project of Beijing Normal University at Zhuhai(jx2023037);Teaching Construction and Reform Project of Beijing Normal University at Zhuhai(jx2024125)

Abstract

Abstract:

As the most thoroughly studied epigenetic modification, DNA methylation-induced silencing of suppressor genes is closely related with the development of cancers. Bioinformatic analysis is an important research tool in the fields of life sciences, medicine, and so on. To introduce the application of bioinformatic analysis in cancer epigenetic research to students and to change the current situation that students usually passively perform experiments during the experimental teaching of genetics, we established a comprehensive and exploratory epigenetic experiment which was integrated with bioinformatic analysis. The implement of this experiment followed the principles of centering on students and cultivating scientific research capabilities. Students selected the genes that were potentially silenced by high DNA methylation in lung adenocarcinoma through bioinformatic analysis and literature review under the guidance of teachers and formulated experimental scheme by their own. They then conducted real-time PCR and methylation-specific PCR to explore whether DNA methylation caused the expression silencing of genes of interest and to identify the DNA methyltransferase responsible for the methylation of genes of interest. To comprehensively assess the capability of students, we designed diversified evaluation methods which intensify the assessment of experiment process. This experiment has the characteristics of interdisciplinarity, comprehensiveness, and inquiry, which can help to cultivate the scientific thinking and practical ability of students.

Key words: DNA methylation, epigenetic, genetics, experiment teaching, comprehensive and exploratory experiment

Sainan An, Huanchun Yang, Shan Jiang, Jingxuan Li, Genfa Zhang. Design and exploration of integrating bioinformatic analysis into comprehensive and exploratory epigenetic experiments[J]. Hereditas(Beijing), 2025, 47(5): 600-608.

Figures/Tables 6

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周次	学时	实验项目	具体内容	实验目的
1	3	实验介绍及理论知识讲解	(1)教师介绍与课程相关理论知识，引导学生提出问题 (2)教师介绍相关数据库及数据分析方法，引导学生确定目的基因 (3)教师介绍实验模块、相关耗材和试剂，引导学生完善实验细节	知识：DNA甲基化的分布和功能等能力：分析数据和绘图；查阅文献；设计实验的能力素质：科研思维及探索精神
2	3	实验方案探讨及细胞处理	(1)实验方案汇报和讨论，确定实验方案 (2)细胞培养与抑制剂处理 (3)实验试剂和耗材准备	能力：准备实验的能力素质：团队协作；实验态度和习惯
3	3	RNA的提取与逆转录	(1)介绍Real-time PCR引物设计原则，指导学生设计引物 (2)提取RNA (3)逆转录	知识：Real-time PCR原理及其应用能力：RNA提取及逆转录素质：实验态度和习惯
4	3	DNA亚硫酸氢盐转化实验	(1)介绍甲基化特异性PCR引物的设计方法和原则 (2)DNA亚硫酸氢盐转化实验	知识：甲基化特异性PCR的原理和方法能力：甲基化特异性PCR实验引物设计素质：探索精神
5	3	Real-time PCR和甲基化特异性PCR实验	(1)Real-time PCR实验 (2)介绍Real-time PCR实验数据分析和注意事项 (3)甲基化特异性PCR实验 (4)介绍科研论文的书写要求和规范以及科研图表的规范	能力：结果分析、科研绘图和科研写作能力素质：团队协作；科研诚信

Design and exploration of integrating bioinformatic analysis into comprehensive and exploratory epigenetic experiments

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