发育调节因子助力作物遗传转化效率提升

doi:10.16288/j.yczz.24-345

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Hereditas(Beijing) ›› 2025, Vol. 47 ›› Issue (9): 992-1006.doi: 10.16288/j.yczz.24-345

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Developmental regulatory factors promote the efficiency of crop genetic transformation

Yingying Xie¹(), Kejian Wang², Yuchun Rao¹(), Yong Huang²()

1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
2. National Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China

Received:2024-12-05 Revised:2025-03-12 Online:2025-04-18 Published:2025-04-18
Contact: Yuchun Rao, Yong Huang E-mail:1485637288@qq.com;ryc@zjnu.cn;huangyong@caas.cn
Supported by:
National Natural Science Foundation of China(32101721);Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-CNRRI);Central Public-Interest Scientific Institution Basal Research Fund(CPSIBRF-CNRRI-202409)

Abstract

Abstract:

Genetic transformation technology is a core tool for generating plants with gain- or loss-of-function in modern biology and agricultural biotechnology. In recent years, developmental regulatory factors (DRs) have shown great potential in enhancing the efficiency of crop genetic transformation. In this review, we systematically examine the main crop genetic transformation methods, including Agrobacterium-mediated transformation and gene gun techniques, and discuss the current challenges in the transformation process, such as low transformation efficiency, strong genotype dependence, and insufficient regeneration capacity. We then provide a detailed discussion of several key developmental regulatory factors and their recent applications in improving transformation efficiency, overcoming genotype dependence, and enhancing regeneration capacity. Additionally, we explore the future prospects of DRs, highlighting their significant potential applications in precision gene editing, functional genomics, synthetic biology, and crop genetic improvement. Through in-depth research on DRs, the bottlenecks in crop genetic transformation are expected to be effectively overcome, thus advancing the further development of crop science and agricultural biotechnology.

Key words: developmental regulatory factors, genetic transformation, regeneration capacity, crop, functional genomics

Yingying Xie, Kejian Wang, Yuchun Rao, Yong Huang. Developmental regulatory factors promote the efficiency of crop genetic transformation[J]. Hereditas(Beijing), 2025, 47(9): 992-1006.

Figures/Tables 2

Table 1

Application of developmental regulatory factors to promote crop genetic transformation and regeneration"

转化与再生	发育调节因子	物种	遗传转化方法	提升效果	参考文献
提高转化效率	ZmWus2	高粱	农杆菌转化法	转化效率最高可达到69.7%	[34]
	BrrWUSa	芜菁	农杆菌转化法	再生效率提高至13%	[36]
	MDBBM	苹果	农杆菌转化法	转化效率最高提高至30%	[38]
	ZmBBM-ZmWUS2	玉米	农杆菌转化法	转化效率最高提高至51.7%	[39]
	ZmBBM-ZmWUS2	高粱	农杆菌转化法	愈伤组织转化频率从1.9%增加到18.3%	[39]
	OsBbm-ZmWUS2	水稻	农杆菌转化法	愈伤组织转化频率从0%增加到43%	[39]
	ZmBBM-ZmWUS2	甘蔗	农杆菌转化法	愈伤组织转化频率最高提高了8.85倍	[39]
	Babyboom-Wuschel	小麦	农杆菌转化法	转化效率为常规方法的5~6倍	[41]
	HvBBM-HvWUS	大麦	农杆菌转化法	再生频率从7.32%增加到24.8%	[42]
克服基因型依赖性	TaWOX5	小麦	农杆菌转化法	转化效率最高可达到97.8%	[44]
	AtGRF5	甜菜	农杆菌转化法	转化效率提高了6倍	[48]
	AtGRF5	西瓜	农杆菌转化法	转化效率从0.88%提高至24.73%	[49]
	TaGRF4-GIF1	小麦	农杆菌转化法	平均再生效率提高了7.8倍	[9]
	TaGRF4-GIF1	黑小麦	农杆菌转化法	再生效率提高至10.7%	[9]
	TaGRF4-GIF1	水稻	农杆菌转化法	再生效率提高了2.1倍	[9]
	ClGRF4-GIF1	西瓜	农杆菌转化法	转化效率提高了近9倍	[50]
	GmGRF3-GIF1	大豆	农杆菌转化法	平均转化效率提高了2.74倍	[51]
	TaGRF4-OsGIF1	高粱	农杆菌转化法	转化效率最高可达到38.28%	[52]
增强再生能力	TaLAX1	小麦	农杆菌转化法	转化频率提高至79.52%	[54]
	ZmBA1	玉米	农杆菌转化法	再生效率从10.59%提高到36.40%	[54]
	GmLAX1	大豆	农杆菌转化法	再生频率从15.68%提高到26.18%	[54]
	ZmWIND1	玉米	农杆菌转化法	转化效率最高提高至37.5%	[64]
	REF1	番茄	农杆菌转化法	再生和转化效率分别提高了19倍和12倍	[65]
	GmREF1	大豆	农杆菌转化法	再生和转化效率分别提高了9倍和5倍	[65]
	TaREF1	小麦	农杆菌转化法	再生和转化效率分别提高了8倍和4倍	[65]
	ZmREF1	玉米	农杆菌转化法	再生和转化效率分别提高了6倍和4倍	[65]
	TaDOF5.6	小麦	农杆菌转化法	转化效率最高提高至50%	[67]
	TaDOF3.4	小麦	农杆菌转化法	转化效率最高提高至55%	[67]
	GhAGL15	棉花	农杆菌转化法	愈伤组织形成率从38.1%增加到65.2%	[72]

Table 1

Fig. 1

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Developmental regulatory factors promote the efficiency of crop genetic transformation

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