Prime editing引导植物基因组精确编辑新局面

doi:10.16288/j.yczz.20-125

[an error occurred while processing this directive]

Hereditas(Beijing) ›› 2020, Vol. 42 ›› Issue (6): 519-523.doi: 10.16288/j.yczz.20-125

• Frontier Focus • Next Articles

Prime editing creates a novel dimension of plant precise genome editing

Ruiying Qin, Pengcheng Wei()

Rice Institute, Anhui Academy of Agriculture Science, Anhui Key Laboratory of Rice Genetics and Breeding, Hefei 230031, China

Received:2020-05-02 Revised:2020-05-20 Online:2020-06-20 Published:2020-05-28
Contact: Wei Pengcheng E-mail:weipengcheng@gmail.com
Supported by:
Supported by the Genetically Modified Breeding Major Projects Nos(2019ZX08010003-001-008);Supported by the Genetically Modified Breeding Major Projects Nos(2016ZX08010-002-008)

Abstract

Abstract:

The precise genome editing has not been well established in plants, largely because of the limited frequency of homology recombination and the delivery barrier of donor templates. Recently, Dr. Caixia Gao’s group from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, developed a series of plant prime editors (PPEs), which mediats the prime editing in the genomes of rice and wheat. The PPE systems are able to generate all 12 kinds of programmable base substitutions, as well desired multiplex nucleotide substitutions and small deletions or insertions without DNA double-strand breaks, thus providing versatile tools for precise plant genome editing. Herein, we introduce the structure and the editing capacity of the PPEs. The attemp on efficiency enhancements of PPEs and other PPEs are also discussed, which may provide a reference for appropriate application of PPEs in plants and also for continuous optimization of the editing tools.

Key words: prime editing, precise editing, CRISPR, genome editing, crop

Ruiying Qin, Pengcheng Wei. Prime editing creates a novel dimension of plant precise genome editing[J]. Hereditas(Beijing), 2020, 42(6): 519-523.

Figures/Tables 1

References 19

[1]	Chen KL, Wang YP, Zhang R, Zhang HW, Gao CX . CRISPR/Cas genome editing and precision plant breeding in agriculture. Annu Rev Plant Bio, 2019,70:667-697.
[2]	Sun YW, Li JY, Xia LQ . Precise genome modification via sequence-specific nucleases-mediated gene targeting for crop improvement. Front Plant Sci, 2016,7:1928.
[3]	Lin QP, Zong Y, Xue CX, Wang SX, Jin S, Zhu ZX, Wang YP, Anzalone AV, Raguram A, Doman JL, Liu DR, Gao CX . Prime genome editing in rice and wheat. Nat Biotechnol, 2020,38:582-585.
[4]	Zong Y, Gao CX . Progress on base editing systems. Hereditas(Beijing), 2019,41(9):777-800.
	宗媛, 高彩霞 , 碱基编辑系统研究进展. 遗传, 2019,41(9):777-800.
[5]	Rees HA, Liu DR . Base editing: precision chemistry on the genome and transcriptome of living cells. Nat Rev Genet, 2018,19(12):770-788.
[6]	Jin S, Zong Y, Gao Q, Zhu ZX, Wang YP, Qin P, Liang CZ, Wang DW, Qiu JL, Zhang F, Gao CX . Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science, 2019 364(6437):292-295.
[7]	Zuo EW, Sun YD, Wei W, Yuan TL, Ying WQ, Sun H, Yuan LY, Steinmetz LM, Li YX, Yang H . Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science, 2019,364(6437):289-292.
[8]	Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR . Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 2019,576(7785):149-157.
[9]	Butt H, Rao GS, Sedeek K, Aman R, Kamel R, Mahfouz M. Engineering herbicide resistance via prime editing in rice. Plant Biotechnol J, 2020, https://doi.org/10.1111/pbi.13399 .
[10]	Hua K, Jiang YW, Tao XP, Zhu JK. Precision genome engineering in rice using prime editing system. Plant Biotechnol J, 2020, https://doi.org/10.1111/pbi.13395 .
[11]	Li HY, Li JY, Chen JL, Yan L, Xia LQ . Precise modifications of both exogenous and endogenous genes in rice by prime editing. Mol Plant, 2020, https://doi.org/10.1016/j.molp.2020.03.011 .
[12]	Tang X, Sretenovic S, Ren QR, Jia XY, Li MK, Fan TT, Yin DS, Xiang SY, Guo YC, Liu L, Zheng XL, Qi YP, Zhang Y . Plant prime editors enable precise gene editing in rice cells. Mol Plant, 2020,13(5):667-670.
[13]	Xu RF, Li J, Liu XH, Shan TF, Qin RY, Wei PC . Development of plant prime-editing systems for precise genome editing. Plant Commun, 2020, .
[14]	Xu W, Zhang CW, Yang YX, Zhao S, Kang GT, He XQ, Song JL, Yang JX . Versatile nucleotides substitution in plant using an improved prime editing system. Mol Plant, 2020,13(5):675-678.
[15]	Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR . Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol, 2018,36(9):843-846.
[16]	Tang X, Zheng XL, Qi YP, Zhang DW, Cheng Y, Tang AT, Voytas DF, Zhang Y . A single transcript CRISPR-Cas9 system for efficient genome editing in plants. Mol Plant, 2016,9(7):1088-1091.
[17]	Xie KB, Minkenberg B, Yang YN . Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci USA, 2015,112(11):3570-3575.
[18]	Li J, Qin RY, Zhang YD, Xu SB, Liu XS, Yang JB, Zhang XQ, Wei PC. Optimizing plant adenine base editor systems by modifying the transgene selection system. Plant Biotechnol J, 2019, .
[19]	Xu W, Yang YX, Liu Y, Kang GT, Wang FP, Li L, Lv XX, Zhao S, Yuan S, Song JL, Wu Y, Feng F, He XQ, Zhang CW, Song W, Zhao JR, Yang JX . Discriminated sgRNAs- based SurroGate system greatly enhances the screening efficiency of plant base-edited cells. Mol Plant, 2020,13(5):169-180.

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Prime editing creates a novel dimension of plant precise genome editing

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 1

References 19

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Mingjiang Chen, Guifu Liu, Yeqing Xiao, Hong Yu, Jiayang Li. Breeding of ZhongKeFaZaoGeng1 by molecular design [J]. Hereditas(Beijing), 2023, 45(9): 829-834.
[2]	Qianwen Lv, Yongfang Yang. The biological functions of peptide signaling in plant and the advances on its utilization for crop improvement [J]. Hereditas(Beijing), 2023, 45(9): 813-828.
[3]	Xiaoping Lian, Guangfu Huang, Yujiao Zhang, Jing Zhang, Fengyi Hu, Shilai Zhang. The discovery and utilization of favorable genes in Oryza longistaminata [J]. Hereditas(Beijing), 2023, 45(9): 765-780.
[4]	Liumei Jian, Yingjie Xiao, Jianbing Yan. De novo domestication: a new way for crop design and breeding [J]. Hereditas(Beijing), 2023, 45(9): 741-753.
[5]	Qingyu Sun, Yang Zhou, Lijuan Du, Mengke Zhang, Jiale Wang, Yuanyuan Ren, Fang Liu. Analysis between macrophage-related genes with prognosis and tumor microenvironment in non-small cell lung cancer [J]. Hereditas(Beijing), 2023, 45(8): 684-699.
[6]	Bingzheng Wang, Chao Zhang, Jiali Zhang, Jin Sun. Conditional editing of the Drosophila melanogaster genome using single transcripts expressing Cas9 and sgRNA [J]. Hereditas(Beijing), 2023, 45(7): 593-601.
[7]	Danni Liu, Haiping Wu, Guohua Zhou. Research progress of visual detection in rapid on-site detection of pathogen nucleic acid [J]. Hereditas(Beijing), 2023, 45(4): 306-323.
[8]	Zhongsheng Wu, Yu Gao, Yongtao Du, Song Dang, Kangmin He. The protocol of tagging endogenous proteins with fluorescent tags using CRISPR-Cas9 genome editing [J]. Hereditas(Beijing), 2023, 45(2): 165-175.
[9]	Meizhen Liu, Liren Wang, Yongmei Li, Xueyun Ma, Honghui Han, Dali Li. Generation of genetically modified rat models via the CRISPR/Cas9 technology [J]. Hereditas(Beijing), 2023, 45(1): 78-87.
[10]	Xiaojun Zhang, Kun Xu, Juncen Shen, Lu Mu, Hongrun Qian, Jieyu Cui, Baoxia Ma, Zhilong Chen, Zhiying Zhang, Zehui Wei. A CRISPR/Cas9-Gal4BD donor adapting system for enhancing homology-directed repair [J]. Hereditas(Beijing), 2022, 44(8): 708-719.
[11]	Chong Zhang, Zixuan Wei, Min Wang, Yaosheng Chen, Zuyong He. Editing MC1R in human melanoma cells by CRISPR/Cas9 and functional analysis [J]. Hereditas(Beijing), 2022, 44(7): 581-590.
[12]	Xue Lv, Bangjie Li, Hanmei Xu. Research progress on high throughput discovery and functional verification of functional micropeptides [J]. Hereditas(Beijing), 2022, 44(6): 478-490.
[13]	Yangjinghui Zhang, Peiyao Chang, Zishu Yang, Yuhang Xue, Xueqi Li, Yang Zhang. Advances in epigenetic modification affecting anthocyanin synthesis [J]. Hereditas(Beijing), 2022, 44(12): 1117-1127.
[14]	Yao Liu, Xianhui Zhou, Shuhong Huang, Xiaolong Wang. Prime editing: a search and replace tool with versatile base changes [J]. Hereditas(Beijing), 2022, 44(11): 993-1008.
[15]	Yuting Han, Bowen Xu, Yutong Li, Xinyi Lu, Xizhi Dong, Yuhao Qiu, Qinyun Che, Ruibao Zhu, Li Zheng, Xiaochen Li, Xu Si, Jianquan Ni. The cutting edge of gene regulation approaches in model organism Drosophila [J]. Hereditas(Beijing), 2022, 44(1): 3-14.