鳜脂蛋白脂酶基因SNP及其与食性驯化相关性分析

doi:10.3724/SP.J.1005.2011.00996

[an error occurred while processing this directive]

HEREDITAS ›› 2011, Vol. 33 ›› Issue (9): 996-1002.doi: 10.3724/SP.J.1005.2011.00996

• en • Previous Articles Next Articles

Polymorphism of SNPs in the lipoprotein lipase (LPL) in Siniperca chuatsi and their association with feed habit domestication

YANG Yu-Hui, LIANG Xu-Fang, FANG Rong, PENG Min-Yan, HUANG Zhi-Dong

College of Life Science and Technology, Jinan University, Guangzhou 510632, China

Received:2010-11-20 Revised:2011-01-13 Online:2011-09-20 Published:2011-09-25

Abstract

Abstract: Sinperca chuatsi usually refuses eating dead bait or man-made feed due to its specific feeding habit. It was showed that some S. chuatsi could be induced to take lifeless bait with domestication gradually after long-term of cultivation. High cost, serious pollution, severe diseases, and other problems of S chuatsi cultivation can be solved effectively by selecting those liable to domestication via molecular marker and treating them with artificial feed on a large scale. Lipoprotein lipase, one of the key enzymes in lipoprotein metabolic processes, provides energy by catalyzing the oxidation of triglycerides lie in the core of chylomicron and very low density lipoprotein (VLDL) into glycerin and fatty acid, and saves energy. In order to search the distribution of the alleles and genotypes of lipoprotein lipase gene (lipoprotein LPL) gene in two feed habit domestication phenotype populations, SNP genetic polymorphisms in 6 and 7 introns and 6, 7, and 8 exons of LPL in S. chuatsi were analyzed by PCR product sequencing method. Three SNPs A25T, G26T, and C29G were detected, two of which were non-synonymous mutations. It was indicated by Chisquare test that the analysis between domesticated and undomesticated populations show no significant difference (P>0.05) between the three SNPs and feed habit. However, diplotype 2 in the two population showed significant difference (P<0.05) by assembling different genotypes of the three SNPs into five diplotypes. Polymorphic analysis of partial fragment of LPL genome in S. chuatsi was accomplished successfully. Therefore, LPL gene can be considered as a candidate gene and genetic marker for feed habit domestication in S. chuatsi, which lays the foundation for the work of molecular marker and selecting breed, which has extensive application prospect.

Key words: lipoprotein lipase, Siniperca chuatsi, single nucleotide polymorphism, feed habit domestication

YANG Yu-Hui, LIANG Xu-Fang, FANG Rong, BANG Min-Yan, HUANG Zhi-Dong. Polymorphism of SNPs in the lipoprotein lipase (LPL) in Siniperca chuatsi and their association with feed habit domestication[J]. HEREDITAS, 2011, 33(9): 996-1002.

References

[1] 梁旭方. 国内外鳜类研究及养殖概况. 水产科技情报, 1996, 23(1): 13-17.
[2] 梁旭方, 俞伏虎, 何炜, 贺锡勤, 黄永川, 蔡书恒. 配合饲料网箱养殖商品鳜的初步研究. 水利渔业, 1995(2): 3-5.
[3] 梁旭方, 贺锡勤. 鲜饲料网箱养殖商品鳜的初步研究. 水利渔业, 1994, (1): 3-4, 11.
[4] 杨凤. 动物营养学 (第二版). 北京: 中国农业出版社, 2007: 76-88.
[5] Wong H, Schotz MC. The lipase gene family. J Lipid Res, 2002, 43(7): 993-999.
[6] Mukherjee M. Human digestive and metabolic lipases-a brief review. J Mol Catal, 2003, 22(5-6): 369-376.
[7] Auwerx J, Leroy P, Schoonjans K. Lipoprotein lipase: recent contributions from molecular biology. Crit Rev Clin Lab Sci, 1992, 29(3-4): 243-268.
[8] 姚煜, 梁旭方, 李光照, 王琳, 刘秀霞. 鳜脂蛋白脂酶和肝脂酶基因结构与组织表达. 中国水产科学, 2009, 16(4): 506-517.
[9] Reymer PWA, Gagné E, Groenemeyer BE, Zhang HF, Forsyth I, Jansen H, Seidell JC, Kromhout D, Lie KE, Kastelein J, Hayden MR. A lipoprotein lipase mutation (Asn291Ser) is associated with reduced HDL cholesterol levels in Premature atherosclerosis. Nat Genet, 1995, 10(1): 28-34.
[10] Chamberlain JC, Thorn JA, Oka K, Galton DJ, Stocks J. DNA polymorphisms at the lipoprotein lipase gene: associations in normal and hypertriglyceridaemic subjects. Atherosclerosis, 1989, 79(1): 85-91.
[11] Jemaa R, Tuzet S, Portos C, Betoulled D, Apfelbaum M, Fumeron F. Lipoprotein lipase gene polymor-phisms: associations with hypertriglyceridemia and body mass index in obese people. Int J Obes Relat Metab Disord, 1995, 19(4): 270-274.
[12] Heizmann C, Kirchgessner T, Kwiterovich PO, Ladias JA, Derby C, Antonarakis SE, Lusis AJ. DNA polymorphism haplotypes of the human lipoprotein lipase gene: possible association with high density lipoprotein levels. Hum Genet, 1991, 86(6): 578-584.
[13] Thorn JA, Chamberlain JC, Alcolado JC, Oka K, Chan L, Stocks J, Galton DJ. Lipoprotein and hepatic lipase gene variants in coronary atherosclerosis. Atherosclerosis, 1990, 85(1): 55-60.
[14] Harbitz I, Kristensen T, Kran S, Davies W. Isolation and sequencing of porcine lipoprotein lipase cDNA and its use in multiallelic restriction fragment length polymorphism detection. Anim Genet, 1992, 23(6): 517-522.
[15] 张依裕, 徐琪, 张海波, 吉文林, 段修军, 陈国宏. 金定鸭LPL基因内含子5多态性对生产性能的影响.畜牧与兽医, 2009, 41(10): 37-40.
[16] Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 1980, 32(3): 314-331.
[17] Nei M. Molecular Population Genetics and Evolution. Translated by Wang JY. Beijing: Agricultural Press, 1983.
[18] Daly MJ, Rioux JD, Schaffner SF, Hudson TJ, Lander ES. High-resolution haplotype structure in the human genome. Nat Genet, 2001, 29(2): 229-232.
[19] Bader JS. The relative power of SNPs and haplotype as genetic markers for association tests. Pharmacogenomics, 2001, 2(1): 11-24.
[20] Clark AG. The role of haplotypes in candidate gene studies. Genet Epidemiol, 2004, 27(4): 321-333.
[21] 刘福平, 白俊杰, 叶星, 李胜杰, 李小慧, 于凌云. 罗非鱼MC4R基因克隆及与其生长相关的SNPs位点. 中国水产科学, 2009, 16(6): 816-823.
[22] Stephens M, Smith NJ, Donnelly P. A new statistical method for haplotype reconstruction from population data. Am J Hum Genet, 2001, 68(4): 978-989.
[23] 刘铮铸, 李祥龙, 巩元芳, 勒晓敏, 冯敏山, 武成. 绵羊MSTN基因内含子2和外显子3部分序列的SNP检测和单倍型分析. 中国畜牧杂志, 2010, 46(7): 9-12.

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Polymorphism of SNPs in the lipoprotein lipase (LPL) in Siniperca chuatsi and their association with feed habit domestication

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Haoyu Wang, Yuhan Hu, Yueyan Cao, Qiang Zhu, Yuguo Huang, Xi Li, Ji Zhang. AI-SNPs screening based on the whole genome data and research on genetic structure differences of subcontinent populations [J]. Hereditas(Beijing), 2021, 43(10): 938-948.
[2]	Yuzhuo Wang, Yiming Zhang, Xiaolian Dong, Xuecai Wang, Jianfu Zhu, Na Wang, Feng Jiang, Yue Chen, Qingwu Jiang, Chaowei Fu. Modification effects of T2DM-susceptible SNPs on the reduction of blood glucose in response to lifestyle interventions [J]. Hereditas(Beijing), 2020, 42(5): 483-492.
[3]	Qing Zhang,Jie Ping,Haoxiang Zhang,Bo Kang, ,Gangqiao Zhou. Genetic association of MKL1 gene polymorphisms with the high-altitude adaptation [J]. Hereditas(Beijing), 2019, 41(7): 634-643.
[4]	Zhe-ye Peng,Zi-jun Tang,Min-zhu Xie. Research progress in machine learning methods for gene-gene interaction detection [J]. Hereditas(Beijing), 2018, 40(3): 218-226.
[5]	Lili Liu, Aiwei Guo, Peifu Wu, Fenfen Chen, Yajin Yang, Qin Zhang. Regulation of VPS28 gene knockdown on the milk fat synthesis in Chinese Holstein dairy [J]. Hereditas(Beijing), 2018, 40(12): 1092-1100.
[6]	Xian Gong, Chao Zhang, Aisa Yiliyasi, Ying Shi, Xuewei Yang, Aosiman Nuersimanguli, Yaqun Guan, Shuhua Xu. A comparative analysis of genetic diversity of candidate genes associated with type 2 diabetes in worldwide populations [J]. Hereditas(Beijing), 2016, 38(6): 543-559.
[7]	Dayan Niu, Weili Yan. The application of genetic risk score in genetic studies of complex human diseases [J]. HEREDITAS(Beijing), 2015, 37(12): 1204-1210.
[8]	Qingfeng Song, Hongxing Zhang, Yilong Ma, Gangqiao Zhou. Fine mapping of complex disease susceptibility loci [J]. HEREDITAS, 2014, 36(1): 2-10.
[9]	LI Qian, LIU Shu-Yuan, LIN Ke-Qin, SUN Hao, YU Liang, HUANG Xiao-Qin, CHU Jia-You, YANG Zhao-Qing. Association between six single nucleotide polymorphisms of EGLN1 gene and adaptation to high-altitude hypoxia [J]. HEREDITAS, 2013, 35(8): 992-998.
[10]	XIA Zheng-Long YU Ju-Hua LI Hong-Xia LI Jian-Lin TANG Yong-Kai REN Hong-Tao ZHU Shuang-Ning. Sequences analysis of jlFABP2 and the correlation between polymorphisms and body weight gain in Cyprinus carpio var. jian [J]. HEREDITAS, 2013, 35(5): 628-636.
[11]	MA Xiao-Jun GUO Hao-Hui HAO Shao-Wen SUN Shou-Xuan YANG Xiao-Chun YU Bo JIN Qun-Hua. Association of single nucleotide polymorphisms (SNPs) in leptin receptor gene with knee osteoarthritis in the Ningxia Hui population [J]. HEREDITAS, 2013, 35(3): 359-364.
[12]	YANG Ying-Zhong WANG Ya-Ping MA Lan DU Yang GE Ri-Li. Genome-wide association study of high-altitude pulmonary edema in Han Chinese [J]. HEREDITAS, 2013, 35(11): 1291-1299.
[13]	LIU Xi-Dong, WANG Zhi-Peng, FAN Hui-Zhong, LI Jun-Ya, GAO Hui-Jiang. Artificial selection for cattle based on high-density SNP markers [J]. HEREDITAS, 2012, 34(10): 1304-1313.
[14]	ZHANG Xiao-Bo, DIAO Zhen-Hong, CHEN Gong-Yan, WANG Jiu-Cun, JIAN Ji, YANG E-Jun, WEI Qiang-Xi, HUANG Jian, LEI Da-Ru. Human chromosome 8p11 (CHRNB3-CHRNA6) region gene polymorphisms and susceptibility to lung cancer in Chinese Han population [J]. HEREDITAS, 2011, 33(8): 886-894.
[15]	ZHANG Zi-Bei, XU Li-Jun, YANG Kang-Juan, XU Liang-Wei, CHENG Tian-Cuan, HAO Ping, WANG Yu-Ping, MENG Fan-Beng. Association between single nucleotide polymorphisms (SNPs) at the promoter of adiponectin gene and essential hypertension in Chinese Korean and Han of Yanbian region [J]. HEREDITAS, 2011, 33(1): 54-59.