一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究

doi:10.3724/SP.J.1005.2009.01.004

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HEREDITAS ›› 2009, Vol. 31 ›› Issue (12): 1241-1247.doi: 10.3724/SP.J.1005.2009.01.004

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Chromosomal localization and development of SNP markers of a serine protease gene in Farrer’s scallop (Chlamys farreri)

HUAN Pin^{1, 2}, ZHANG Xiao-Jun¹, LI Fu-Hua¹, ZHANG Yang^{1, 3}, ZHAO Cui¹, LIU Bao-Zhong¹, XIANG Jian-Hai¹

1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;
3. Department of Soil & Crop Sciences, College Station, Texas A & M University, TX 77843-2472, USA

Received:2009-03-06 Revised:2009-10-09 Online:2009-12-10 Published:2009-11-17
Contact: XIANG Jian-Hai E-mail:jhxiang@ms.qdio.ac.cn

Abstract

Abstract:

Disease control is a main objective in the culture of Farrer’s scallop (Chalmys farreri). Many investigations have focused on the innate immune system of this species. Serine proteases amplify signals in many immune-related pathways and thus play a crucial role in innate immune system. Recently, studies on serine protease in C. farreri were mainly on sequence analysis and expression profile, but no study on gene localization was reported. In this study, a BAC clone (CFB040H03) that contains a serine protease gene of C. farreri was located on the long arms of a pair of homologous chromosomes by BAC-FISH technique. Six SNPs were found inside the serine protease gene using direct sequencing of the PCR products. The chromosomal localization of serine protease gene would provide fundamental support for further research on this gene. The SNPs obtained can be mapped to the genetic linkage map and is helpful to the construction of the integration map of C. farreri.

Key words: Chalmys farreri, serine protease, fluorescence in situ hybridization (FISH), chromosome, single nucleotide polymorphism (SNP)

HUAN Pin, ZHANG Xiao-Jun, LI Fu-Hua, ZHANG Xiang, DIAO Cui, LIU Bao-Zhong, XIANG Jian-Hai. Chromosomal localization and development of SNP markers of a serine protease gene in Farrer’s scallop (Chlamys farreri)[J]. HEREDITAS, 2009, 31(12): 1241-1247.

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Chromosomal localization and development of SNP markers of a serine protease gene in Farrer’s scallop (Chlamys farreri)

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