[an error occurred while processing this directive]

HEREDITAS ›› 2010, Vol. 32 ›› Issue (3): 219-228.doi: 10.3724/SP.J.1005.2010.00219

• en • Previous Articles     Next Articles

Genomic analysis of DNA-protein interaction by Chromatin immunoprecipitation

LI Min-Li1, WANG Wei1, LU Zu-Hong1,2   

  1. 1. The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; 
    2. Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing 210096, China
  • Received:2009-06-23 Revised:2009-08-27 Online:2010-03-20 Published:2010-03-18
  • Contact: LU Zu-Hong E-mail:zhlu@seu.edu.cn

Abstract:

Chromatin immunoprecipitation (ChIP) is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo. ChIP coupled with high density microarray (ChIP-chip) or high-throughput sequencing (ChIP-Seq) has generated large amount of data and expected to allow the development of a network describing the cellular transcriptional regulation. Here, we reviewed the ChIP, ChIP-chip, and ChIP-Seq techniques as well as their perspectives. Focus is given to data analysis of ChIP-Seq and the applications of ChIP-chip and ChIP-Seq.

Key words: genome, ChIP-Seq, ChIP-chip