蓝狐-卫星DNA的克隆及序列分析

doi:10.1360/yc-007-1504

遗传 ›› 2007, Vol. 29 ›› Issue (12): 1504-1508.doi: 10.1360/yc-007-1504

蓝狐-卫星DNA的克隆及序列分析

闫守庆¹, 祝万菊¹, 张雪梅¹, 李冰¹, 孙金海^{1, 2}

1. 吉林大学畜牧兽医学院, 长春 130062; 2. 青岛农业大学动物科技学院, 青岛 266109

收稿日期:2007-05-10 修回日期:2007-06-28 出版日期:2007-12-10 发布日期:2007-12-10
通讯作者: 闫守庆

Cloning and sequence analysis of alpha satellite DNA in arctic fox

YAN Shou-Qing¹, ZHU Wan-Ju¹, ZHANG Xue-Mei¹, LI Bing¹, SUN Jin-Hai^1,2

1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 2. College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China

Received:2007-05-10 Revised:2007-06-28 Online:2007-12-10 Published:2007-12-10
Contact: YAN Shou-Qing

摘要/Abstract

摘要：

利用限制性内切酶酶切蓝狐基因组, 经琼脂糖凝胶电泳, 对特异性亮带进行克隆、测序及序列分析。结果获得42个卫星DNA序列, 该卫星DNA单体大小为737 bp, G+C含量为51.9%, 单体之间同源性为91%~97%; 每个单体由3个约245 bp的亚重复串联构成, 亚重复之间的同源性为49%~55%; 在物种进化过程中, 该卫星DNA有G+C含量逐渐降低而A+T含量逐渐上升的趋势; 该卫星DNA为犬科动物种属所特有, 与犬着丝粒相关卫星DNA为同类卫星DNA, 同源性为74%, 命名为α-卫星DNA。

关键词: 卫星DNA, 蓝狐, 犬科动物

Abstract:

Genomic DNAs from Arctic fox were cleaved with restriction enzymes. The prominent ethidium-bromide stanined bands were cloned and sequenced after separation of DNA fragments by agarose gel electrophoresis. Forty-two satellite sequences were obtained by sequencing. Sequence analyses revealed that the satellite monomer in length was 737 bp, with an average G+C content of 51.9%. There were 91%-97% homology among the monomers, and the monomer consisted of three subrepeats of approximately 245 bp in length with 51%-54% homology. The satellite DNAs were specific for Canidae, and had 74% homology to the dog centromere-associated nucleotide sequences. In order to distinguish from another satellite DNAs of canidae genome, the satellite was designated as alpha satellite DNA.

闫守庆，祝万菊，张雪梅，李冰，孙金海. 蓝狐-卫星DNA的克隆及序列分析[J]. 遗传, 2007, 29(12): 1504-1508.

YAN Shou-Qing, ZHU Wan-Ju, ZHANG Xue-Mei, LI Bing, SUN Jin-Hai. Cloning and sequence analysis of alpha satellite DNA in arctic fox[J]. HEREDITAS, 2007, 29(12): 1504-1508.

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蓝狐-卫星DNA的克隆及序列分析

Cloning and sequence analysis of alpha satellite DNA in arctic fox

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