玉米近缘种中玉米着丝粒序列的FISH检测

doi:10.3724/SP.J.1005.2010.00264

遗传 ›› 2010, Vol. 32 ›› Issue (3): 264-270.doi: 10.3724/SP.J.1005.2010.00264

玉米近缘种中玉米着丝粒序列的FISH检测

佘朝文^{1, 2},蒋向辉^{1, 2},宋运淳³,刘伟¹

1. 怀化学院生命科学系, 湖南怀化 418008;
2. 怀化学院民族药用植物资源研究与利用湖南省重点实验室, 湖南怀化 418008;
3. 武汉大学植物发育生物学教育部重点实验室, 武汉 430072

收稿日期:2009-08-10 修回日期:2009-10-20 出版日期:2010-03-20 发布日期:2010-03-15
通讯作者: 佘朝文 E-mail:shechaowen@tom.com
基金资助:
湖南省教育厅科研基金项目(编号：07C502)资助

Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization

SHE Chao-Wen^{1, 2},JIANG Xiang-Hui^{1, 2},SONG Yun-Chun³, LIU Wei¹

1. Department of Life Sciences, Huaihua University, Huaihua 418008, China;
2. Key Laboratory of Study and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua 418008, China;
3. Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China

Received:2009-08-10 Revised:2009-10-20 Online:2010-03-20 Published:2010-03-15
Contact: SHE Chao-Wen E-mail:shechaowen@tom.com

摘要/Abstract

摘要：

为分析玉米着丝粒卫星DNA(CentC)和着丝粒反转录转座子(CRM)在玉米种的亚种及近缘种中的保守性,采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高粱中的存在和分布。CentC、CRM探针在墨西哥玉米、二倍体多年生类玉米和多年生类玉米的所有染色体的着丝粒区产生了强或较强的杂交信号, 而且不同染色体的杂交信号的强度存在差异, 表明两种玉米着丝粒重复序列在不同着丝粒中的数量不同; 此外, 部分着丝粒中的CentC和CRM信号的强度存在差异, 不完全重叠。CentC、CRM探针仅在摩擦禾的多数染色体的着丝粒区产生了弱的杂交信号。在薏苡和高粱中仅测检到主要分布在着丝粒区的较强或强的CRM信号。这些结果表明, CentC在玉米种的亚种间及玉蜀黍属的物种间高度保守, 在与玉蜀黍属亲缘关系最近的摩擦禾属物种中也具有较高的保守性; CRM在与玉蜀黍属亲缘关系较近和较远的禾本科种属中都具有保守性。

关键词: 玉蜀黍属, 着丝粒, 玉米着丝粒卫星DNA, 玉米着丝粒反转录转座子, 荧光原位杂交

Abstract:

In order to analyze the conservation of maize centromeric satellite DNA (CentC) and centromeric retrotransposon (CRM) in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization (FISH) was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor. In Z. mays ssp. mexicana, Z. diploperennis, and Z. perennis, both CentC and CRM probes produced strong or relatively strong signals in the centromeric regions of all chromosomes. There was an obvious variation in the intensity of hybridization signals on different chromosomes, indicating that different centromeres have different amounts of CentC and CRM sequences. In some centromeres, the intensity of CentC signals differed from that of CRM signals and was free from overlapping. In T. dactyloides, only weak CentC and CRM signals were detected in the centromeric regions of most chromosomes, while in C. lacryma-jobi and S. bicolor only relatively strong or strong CRM signals primarily located in the centromeric regions were detected. This result indicates that CentC is highly conserved among the subspecies of Z. mays and the species of Zea, and has high conservation in Tripsacum, a genus that is most closely related to Zea, and CRM is conserved among the species of grass family either closely or distantly related to Zea.

Key words: Zea, centromere, CentC, CRM, fluorescence in situ hybridization

佘朝文，蒋向辉，宋运淳，刘伟. 玉米近缘种中玉米着丝粒序列的FISH检测[J]. 遗传, 2010, 32(3): 264-270.

SHE Chao-Wen, JIANG Xiang-Hui, SONG Yun-Chun, LIU Wei. Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization[J]. HEREDITAS, 2010, 32(3): 264-270.

参考文献

[1] Jiang J, Birchler JA, Parrott WA, Dawe RK. A molecular view of plant centromeres. Trends Plant Sci, 2003, 8(12): 570–575.

[2] 佘朝文, 宋运淳. 植物着丝粒结构和功能的研究进展. 遗传, 2006, 28(12): 1597–1606.

[3] Cheng Z, Dong F, Langdon T, Ouyang S, Buell CR, Gu M, Blattner FR, Jiang J. Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell, 2002, 14(8): 1691–1704.

[4] Zhong CX, Marshall JB, Topp C, Mroczek R, Kato A, Nagaki K, Birchler JA, Jiang JM, Dawe RK. Centromeric retroelements and satellites interact with maize kineto-chore protein CENH3. Plant Cell, 2002, 14(11): 2825–2836.

[5] Nagaki K, Talbert PB, Zhong CX, Dawe RK, Henikoff S, Jiang, JM. Chromatin immunoprecipitation reveals that the 180 bp satellite repeat is the key functional DNA ele-ment of Arabidopsis thaliana centromeres. Genetics, 2003, 163(3): 1221–1225.

[6] Nagaki K, Cheng Z, Ouyang S, Talbert PB, Kim M, Jones KM, Henikoff S, Buell CR, Jiang J. Sequencing of a rice centromere uncovers active genes. Nat Genet, 2004, 36(2): 138–145.

[7] Nagaki K, Kashihara K, Murata M. Visualization of dif-fuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea. Plant Cell, 2005, 17(7): 1886–1893.

[8] Nagaki K, Murata M. Characterization of CENH3 and centromere-associated DNA sequences in sugarcane. Chromosome Res, 2005, 13(2): 195–203.

[9] Gindullis F, Desel C, Galasso I, Schmidt T. The large- scale organization of the centromeric region in Beta spe-cies. Genome Res, 2001, 11(2): 253–265.

[10] Jin WW, Melo JR, Nagaki K, Talbert PB, Henikoff S, Dawe RK, Jiang J. Maize centromeres: organization and functional adaptation in the genetic background of oat. Plant Cell, 2004, 16(3): 571–581.

[11] Kamm A, Schmidt T, Heslop-Harrison JS. Molecular and physical organization of highly repetitive, undermethy-lated DNA from Pennisetum glaucum. Mol Gen Genet, 1994, 244(4): 420–425.

[12] Kamm A, Galasso I, Schmidt T, Heslop-Harrison JS. Analysis of a repetitive DNA family from Arabidopsis arenosa and relationship between Arabidopsis species. Plant Mol Biol, 1995, 27(5): 853–862.

[13] Harrison GE, Heslop-Harrison JS. Centromeric repetitive DNA sequences in the genus Brassica. Theor Appl Genet, 1995, 90(2): 157–165.

[14] Miller JT, Jackson SA, Nasuda S, Gill BS, Wing RA, Jiang J. Cloning and characterization of a centromere-specific re-petitive DNA element from Sorghum bicolor. Theor Appl Genet, 1998, 96(6–7): 832–839.

[15] Hass BL, Pires JC, Porter R, Phillips RL, Jackson SA. Comparative genetics at the gene and chromosome levels between rice (Oryza sativa) and wildrice (Zizania palustris). Theor Appl Genet, 2003, 107(5): 773–782.

[16] Zhang W, Yi C, Bao W, Liu B, Cui J, Yu H, Cao X, Gu M, Liu M, Cheng Z. The transcribed 165-bp CentO satellite is the major functional centromeric element in the wild rice species Oryza punctata. Plant Physiol, 2005, 139(1): 306–315.

[17] Lee HR, Zhang W, Langdon T, Jin W, Yan H, Cheng Z, Jiang J. Chromatin immunoprecipitation cloning reveals rapid evolutionary patterns of centromeric DNA in Oryza species. Proc Natl Acad Sci USA, 2005, 102(33): 11793–11798.

[18] Lim KB, Yang TJ, Hwang YJ, Kim JS, Park JY, Kwon SJ, Kim J, Choi BS, Lim MH, Jin M, Kim HI, de Jong H, Bancroft I, Lim Y, Park BS. Characterization of the cen-tromere and peri-centromere retrotransposons in Brassica rapa and their distribution in related Brassica species. Plant J, 2007, 49(2): 173–183.

[19] Menzel G, Dechyeva D, Wenke T, Holtgräwe D, Weis-shaar B, Schmidt T. Diversity of a complex centromeric satellite and molecular characterization of dispersed se-quence families in sugar beet (Beta vulgaris). Ann Bot (Lond), 2008, 102(4): 521–530.

[20] Aragon-Alcaide L, Miller T, Schwarzacher T, Reader S, Moore G. A cereal centromeric sequence. Chromosoma, 1996, 105(5): 261–268.

[21] Jiang J, Nasuda S, Dong F, Scherrer CW, Woo SS, Wing RA, Gill BS, Ward DC. A conserved repetitive DNA ele-ment located in the centromeres of cereal chromosomes. Proc Natl Acad Sci USA, 1996, 93(24): 14210–14213.

[22] Presting GG, Malysheva L, Fuchs J, Schubert I. A Ty3/gypsy retrotransposon-like sequence localizes to the centromeric regions of cereal chromosomes. Plant J, 1998, 16(6): 721–728.

[23] Langdon T, Seago C, Mende M, Leggett M, Thomas H, Forster JW, Jones RN, Jenkins G. Retrotransposon evolu-tion in diverse plant genomes. Genetics, 2000, 156(1): 313–325.

[24] Ananiev EV, Phillips RL, Rines HW. Chromosome-specific molecular organization of maize (Zea mays L.) centro-meric regions. Proc Natl Acad Sci USA, 1998, 95(22): 13073–13078.

[25] Lamb JC, Birchler JA. Retroelement genome painting: cyto-logical visualization of retroelement expansions in the genera Zea and Tripsacum. Genetics, 2006, 173(2): 1007–1021.

[26] Song YC, Liu LH, Ding Y, Tian XB, Yao Q, Meng L, He CR, Xu MS. Comparisons of G-banding patterns in six species of the Poaceae. Hereditas, 1994, 121(1): 31–38.

[27] Nagaki K, Song J, Stupar RM, Parokonny AS, Yuan Q, Ouyang S, Liu J, Hsiao J, Jones KM, Dawe RK, Buell CR, Jiang J. Molecular and cytological analyses of large tracks of centromeric DNA reveal the structure and evolutionary dynamics of maize centromeres. Genetics, 2003, 163(2): 759–770.

[28] She CW, Liu JY, Song YC. CPD staining: an effective technique for detection of NORs and other GC-rich chro-mosomal regions in plants. Biotech Histochem, 2006, 81(1): 13–21.

[29] Bao W, Zhang W, Yang Q, Zhang Y, Han B, Gu M, Xue Y, Cheng Z. Diversity of centromeric repeats in two closely related wild rice species, Oryza officinalis and Oryza rhizomatis. Mol Genet Genomics, 2006, 275(5): 421–430.

[30] Zwick MS, Islam-Faridi MN, Zhang HB, Hodnett GL, Gomez MI, Kim JS, Price HJ, Stelly DM. Distribution and sequence analysis of the centromere-associated repetitive element CEN38 of Sorghum bicolor (Poaceae). Am J Bot, 2000, 87(12): 1757–1764.

[31] Doebley JF, Iltis HH. Taxonomy of Zea. 1. Subgeneric classification with key to taxa. Am J Bot, 1980, 67(6): 982–993.

[32] Iltis HH, Doebley JF. Taxonomy of Zea (Gramineae). 2. Subspecific categories in the Zea mays complex and a generic synopsis. Am J Bot, 1980, 67(6): 994–1004.

[33] Meyers BC, Tingey SV, Morgante M. Abundance, distri-bution, and transcriptional activity of repetitive elements in the maize genome. Genome Res, 2001, 11(10): 1660–1676.

[34] 刘纪麟. 玉米育种学. 北京: 农业出版社, 1991, 2–11.

[35] Kellogg EA, Watson L. Phylogenetic studies of a large data set. I. Bambusoideae, Andropogodeae and Pooideae (Gramineae). Bot Rev, 1993, 59(4): 273–343.

[36] Hilton H, Gaut BS. Speciation and domestication in maize and its wild relatives: evidence from the globulin-1 gene. Genetics, 1998, 150(2): 863–872.

[37] Swigonova Z, Lai J, Ma J, Ramakrishna W, Llaca V, Ben-netzen J, Messing J. Close split of sorghum and maize ge-nome progenitors. Genome Res, 2004, 14(10A): 1916–1923.

[38] Liu Z, Yue W, Li D, Wang RR, Kong X, Lu K, Wang G, Dong Y, Jin W, Zhang X. Structure and dynamics of retrotransposons at wheat centromeres and pericentro-meres. Chromosoma, 2008, 117(5): 445–456.

[39] Nagaki K, Neumann P, Zhang D, Ouyang S, Buell CR, Cheng Z, Jiang J. Structure, divergence, and distribution of the CRR centromeric retrotransposon family in rice. Mol Biol Evol, 2005, 22(4): 845–855.

[40] Sharma A, Presting GG. Centromeric retrotransposon lineages predate the maize/rice divergence and differ in abundance and activity. Mol Genet Genomics, 2008, 279(2): 133–147.

[41] Ma J, Jackson SA. Retrotransposon accumulation and sat-ellite amplification mediated by segmental duplication fa-cilitate centromere expansion in rice. Genome Res, 2006, 16(2): 251–259.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

玉米近缘种中玉米着丝粒序列的FISH检测

Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	师明磊,赖维莉,易天红,柯潇,赵志虎. 应用荧光原位杂交技术检测康柏西普基因在CHO细胞染色体上的整合状态[J]. 遗传, 2017, 39(4): 326-332.
[2]	郝佳洁, 王春丽, 顾文跃, 程潇钰, 张钰, 徐昕, 蔡岩, 王明荣. 利用染色体区段混合BAC探针鉴定食管癌细胞中的染色体畸变[J]. 遗传, 2014, 36(6): 558-565.
[3]	戴建国，郑慧华，张平. 染色体移动复合物的结构、定位与功能[J]. 遗传, 2011, 33(6): 576-584.
[4]	汤佳立，戚大石，张俞，刘慧娟，孙健英，曹清河，马代夫，李宗芸. 荧光原位杂交技术分析栽培种甘薯(Ipomoea batatas cv.Xushu No.18)染色体[J]. 遗传, 2010, 32(2): 177-182.
[5]	谢莉，韩永华，李冬郁，曾艳华. FISH分析栽培高粱×拟高粱、甜高粱×拟高粱的染色体行为[J]. 遗传, 2009, 31(4): 420-425.
[6]	康维，姚汉清，房丽丽，蔡岩，韩亚铃，徐昕，张钰，贾雪梅，王明荣 . 食管鳞癌3、8、10、20和Y染色体的非整倍性分析[J]. 遗传, 2009, 31(3): 255-260.
[7]	郇聘，张晓军，李富花，张洋，赵翠，刘保忠，相建海. 一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究[J]. 遗传, 2009, 31(12): 1241-1247.
[8]	陆坤，徐柱，刘朝，张学勇. St基因组中的CRW同源序列在偃麦草中的FISH分析[J]. 遗传, 2009, 31(11): 1141-1148.
[9]	卢洪涌，崔英霞，史轶超，夏欣一，杨滨，姚兵，黄宇烽. 18q部分单体患儿的细胞和分子遗传学研究[J]. 遗传, 2008, 30(8): 991-995.
[10]	杨先泉，赵勤，傅体华. 脉孢霉两对基因顺序四分子分析[J]. 遗传, 2008, 30(6): 801-806.
[11]	裔传灯，龚志云，梁国华，王飞华，汤述翥，顾铭洪. 稻属不同染色体组着丝粒BAC克隆的分离和鉴定[J]. 遗传, 2007, 29(7): 851-858.
[12]	徐延浩，杨飞，程有林，马璐，王建波，李立家. 45S rDNA和5S rDNA在南瓜、丝瓜和冬瓜染色体上的比较定位[J]. 遗传, 2007, 29(5): 614-614―620.
[13]	廖进秋，杨瑞武，周永红，辻本壽. 波兰小麦和矮兰麦45S rDNA和5S rDNA基因位点FISH分析[J]. 遗传, 2007, 29(4): 449-454.
[14]	兰添颖，刘博，董凤平，陈瑞阳，李秀兰，陈成彬. 菠菜rDNA及端粒多色荧光原位杂交分析[J]. 遗传, 2007, 29(11): 1405-1405―1408.
[15]	韩亚玲，冯彦斌，罗曼莉，徐昕，蔡岩，王明荣. 人食管癌EC9706单克隆的分离培养及其生物学特性的研究[J]. 遗传, 2007, 29(11): 1331-1335.