遗传 ›› 2020, Vol. 42 ›› Issue (9): 870-881.doi: 10.16288/j.yczz.20-189

• 综述 • 上一篇    下一篇

用于临床新型冠状病毒核酸检测的分子诊断新技术

谢春梅1, 武海萍1, 马雪萍1, 周国华1,2()   

  1. 1. 南京大学医学院附属金陵医院(东部战区总医院)临床药学科,南京 210002
    2. 南方医科大学药学院,广州 510515
  • 收稿日期:2020-06-22 修回日期:2020-08-19 出版日期:2020-09-20 发布日期:2020-09-04
  • 通讯作者: 周国华 E-mail:ghzhou@nju.edu.cn
  • 基金资助:
    国家自然科学基金项目编号(61871403);江苏省杰出青年基金项目资助编号(BK20180005)

New molecular diagnostic technologies for clinical detection of SARS-CoV-2

Xie Chunmei1, Wu Haiping1, Ma Xueping1, Zhou Guohua1,2()   

  1. 1. Department of Clinical Pharmacy, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China
    2. School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China
  • Received:2020-06-22 Revised:2020-08-19 Online:2020-09-20 Published:2020-09-04
  • Contact: Zhou Guohua E-mail:ghzhou@nju.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China No(61871403);Jiangsu Provincial Science Fund for Distinguished Young Scholars No(BK20180005)

摘要:

目前新型冠状病毒肺炎(corona virus disease 2019, COVID-19)在全球仍呈现大流行趋势,该病潜伏期长、传染性强,给全世界人民的健康安全带来严重威胁。新型冠状病毒(severe acute respiratory syndrome coronavirus 2, SARS-CoV-2)核酸检测作为COVID-19确诊的重要依据之一,在疫情防控工作中发挥了巨大的作用。截至2020年8月17日,国家药品监督管理局已应急批准15个新型冠状病毒核酸检测试剂盒,其中10个试剂盒是以逆转录-实时荧光定量PCR (RT-qPCR)为主要技术原理,其余试剂盒分别采用了不同于RT-qPCR的5种分子诊断技术。本文对上述检测试剂盒所使用的技术原理、反应时间、优缺点等方面进行了介绍,以期为COVID-19及类似传染病的快速筛查、确诊、防控提供更多的方案思路。

关键词: 新型冠状病毒, 核酸检测, 分子诊断

Abstract:

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing pandemic of new coronavirus pneumonia (corona virus disease 2019, COVID-19). The virus has a long incubation period and strong infectivity, which poses a major threat to global health and safety. Detection of SARS-CoV-2 nucleic acid lies at the center of rapid detection of COVID-19, which is instrumental for mitigation of the ongoing pandemic. As of August 17, 2020, The National Medical Products Administration in China has approved 15 new coronavirus nucleic acid detection kits, 10 kits of which are based on reverse transcription-real-time quantitative PCR (RT-qPCR) technology. The remaining kits use five molecular diagnostic technologies different from RT-qPCR. This article reviews the principles, reaction time, advantages and disadvantages of above 15 detection kits, in order to provide references for rapid screening, diagnosis, prevention and control of COVID-19 and similar infectious diseases.

Key words: novel coronavirus, nucleic acid detection, molecular diagnostic technology