胡萝卜(Daucus carota L.)中主要胡萝卜素和番茄红素含量的QTL分析

doi:10.3724/SP.J.1005.2010.01290

遗传 ›› 2010, Vol. 32 ›› Issue (12): 1290-1295.doi: 10.3724/SP.J.1005.2010.01290

胡萝卜(Daucus carota L.)中主要胡萝卜素和番茄红素含量的QTL分析

欧承刚, 邓波涛, 鲍生有, 赵志伟, 胡鸿, 庄飞云, 茅淑敏

中国农业科学院蔬菜花卉研究所, 农业部园艺作物遗传改良重点开放实验室, 北京100081

收稿日期:2010-04-10 修回日期:2010-07-07 出版日期:2010-12-20 发布日期:2010-12-20
通讯作者: 庄飞云 E-mail:fy_zhuang@caas.net.cn
基金资助:
国家科技支撑项目(编号：2006BAD13B06), 农业部948项目(编号：2006-G13), 中央级公益性科研院所基本科研业务费专项(编号：2060302-2-09)和公益性行业(农业)科研专项(编号：200903016)资助

QTL mapping for contents of main carotenes and lycopene in carrot (Daucus carota L.)

OU Cheng-Gang, DENG Bo-Tao, BAO Sheng-You, ZHAO Zhi-Wei, HU Hong, ZHUANG Fei-Yun, MAO Shu-Min

Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China

Received:2010-04-10 Revised:2010-07-07 Online:2010-12-20 Published:2010-12-20
Contact: ZHUANG Fei-Yun E-mail:fy_zhuang@caas.net.cn

摘要/Abstract

摘要： 以高胡萝卜素自交系P50006和HCM A.C.为亲本构建的F₂群体为作图群体, 对胡萝卜中α-胡萝卜素、β-胡萝卜素、总胡萝卜素和番茄红素含量进行QTL定位及遗传分析。结果表明, α、β-胡萝卜素、总胡萝卜素和番茄红素含量的广义遗传力分别为0.75、0.50、0.31和0.93。遗传图谱包含91个SRAP(Sequence-related amplified polymorphism)标记, 分布于9个连锁群, 总长度502.9 cM, 标记间平均距离5.5 cM。除α-胡萝卜素含量外, α-胡萝卜素、总胡萝卜素和番茄红素含量分别检测到1个主效QTL, 均为加性遗传效应, 分别解释表型变异为12.79%、12.87%和14.61%。此外, β-胡萝卜素和番茄红素含量还分别检测到1对上位性QTL, 最大遗传效应分别为显性×加性互作和显性×显性互作, 分别解释表型变异为15.1%和6.5%。文章中与QTL连锁的分子标记可用于高胡萝卜素、番茄红素的种质筛选和聚合育种。

关键词: 胡萝卜, 胡萝卜素, 番茄红素, QTL分析

Abstract: An F₂ population derived from two carrot inbred lines, P50006 and HCM A.C. with high carotene accumulation, was developed and used to map and analyze quantitative trait locus (QTL) associated with the accumulation of α and β-carotene, total carotene and lycopene. Broad-sense heritabilities of these traits were 0.75, 0.50, 0.31, and 0.93, respectively. A genetic map with 91 SRAP (Sequence-related amplified polymorphism) markers was developed, which spanned 502.9 cM in 9 linkage groups with a mean marker interval of 5.5 cM. Mixed-model-based composite interval mapping was performed to analyze QTL and epistasis effects. One major QTL each for β-carotene, total carotene and lycopene accumulation were detected which can explain 12.79%, 12.87%, and 14.61% of total phenotypic variations, respectively. Additive genetic variance was primarily responsible for genetic variability in all three major QTL. In addition, a pair of epistasis QTL for β-carotene and lycopene accumulation was detected, which were able to explain 15.1% and 6.5% of total phenotypic variation, respectively. The dominant × additive and dominant × dominant interaction variance were primary epistasis effect for β-carotene and lycopene. These SRAP markers linked to QTL could be used in selection or QTL pyramiding for high carotene and lycopene content in carrot breeding.

Key words: carrot, carotene, lycopene, QTL mapping

欧承刚，邓波涛，鲍生有，赵志伟，胡鸿，庄飞云，茅淑敏. 胡萝卜(Daucus carota L.)中主要胡萝卜素和番茄红素含量的QTL分析[J]. 遗传, 2010, 32(12): 1290-1295.

OU Cheng-Gang, DENG Bei-Chao, BAO Sheng-Wei, DIAO Zhi-Wei, HU Hong, PENG Fei-Yun, MAO Chu-Min. QTL mapping for contents of main carotenes and lycopene in carrot (Daucus carota L.)[J]. HEREDITAS, 2010, 32(12): 1290-1295.

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胡萝卜(Daucus carota L.)中主要胡萝卜素和番茄红素含量的QTL分析

QTL mapping for contents of main carotenes and lycopene in carrot (Daucus carota L.)

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