多效性基因Ghd7调控水稻剑叶面积

doi:10.3724/SP.J.1005.2012.00901

遗传 ›› 2012, Vol. 34 ›› Issue (7): 901-906.doi: 10.3724/SP.J.1005.2012.00901

多效性基因Ghd7调控水稻剑叶面积

谈聪, 翁小煜, 鄢文豪, 白旭峰, 邢永忠

华中农业大学作物遗传改良国家重点实验室, 武汉 430070

收稿日期:2012-02-09 修回日期:2012-04-06 出版日期:2012-07-20 发布日期:2012-07-25
通讯作者: 邢永忠 E-mail:yzxing@mail.hzau.edu.cn
基金资助:
国家自然科学基金项目(编号：30971749)和转基因专项(编号：2009ZX08009-103B)资助

Ghd7, a pleiotropic gene controlling flag leaf area in rice

TAN Cong, WENG Xiao-Yu, YAN Wen-Hao, BAI Xu-Feng, XING Yong-Zhong

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

Received:2012-02-09 Revised:2012-04-06 Online:2012-07-20 Published:2012-07-25

摘要/Abstract

摘要： 光合作用是植物的唯一能量来源, 剑叶是水稻开花后进行光合作用的主要部位。Ghd7是一个多效性产量基因, 能显著提高水稻产量。为了研究Ghd7对水稻剑叶形态的遗传效应, 文章利用一个包含190个家系的BC₂F₂群体对水稻剑叶长度(FLL)、剑叶宽(FLW)和剑叶面积(FLA)进行QTL定位分析。在BC₂F₂群体, FLL、FLW和FLA性状表型值均显示为双峰分布, 符合孟德尔单基因分离比, 并均与每穗实粒数呈现显著正相关。在第7染色体上RM3859和C39分子标记间定位到FLL、FLW和FLA的 QTL, 分别解释变异的73.3%、62.3%和71.8%, 均与Ghd7共分离。以珍汕97为轮回亲本, 特青和明恢63分别为供体亲本, 获得两个Ghd7近等基因系NIL(MH63)和NIL(TQ), FLL、FLW和FLA表型值均比珍汕97显著提高。另外, 超表达Ghd7的合江19转基因植株的FLL、FLW和FLA表型值分别比合江19增加了8.9 cm、0.5 cm和17.8 cm²。这些结果表明Ghd7对调控剑叶面积起重要作用。

关键词: 近等基因系, QTL分析, 剑叶长, 剑叶宽, 转基因单株

Abstract: Photosynthesis is the unique source of energy for plant. Flag leaf contributed the majority of photosynthate after rice flowering. Ghd7 is a pleiotropic gene, which can significantly increase rice production. In order to study the genetic effects of Ghd7 on the flag leaf morphology, we made quantitative trait locus (QTL) analysis for flag leaf length (FLL), flag leaf width (FLW), and flag leaf area (FLA) using a Ghd7-BC₂F₂ popu-lation of 190 plants. In the BC₂F₂ population, the frequency distribution of FLL, FLW, and FLA were bimodal and in agreement with single Mendelian segregation ratio (3:1). FLL, FLW, and FLA were positively correlated with grains per panicle in the population. One QTL was mapped to the interval between markers RM3859 and C39 on chromosome 7, which explained 73.3%, 62.3%, and 71.8% of the variations for FLL, FLW, and FLA, and co-segregated with Ghd7. Two near-isogenic lines of NIL (mh7) and NIL (tq7) were developed using Zhenshan 97 as the recurrent parent and Minghui 63 and Teqing as the donor parent, respectively. Both NILs significantly increased the phenotypic values of FLL, FLW, and FLA as compared with Zhenshan 97. FLL, The values of FLW and FLA for Ghd7 over-expression transgenic plants were 8.9 cm, 0.5 cm, and 17.8 cm² larger than its recipient Hejiang 19. These results demonstrated that Ghd7 plays an important role in controlling the flag leaf area in rice.

Key words: near-isogenic line, QTL analysis, flag leaf length, flag leaf width, transgenic plant

谈聪，翁小煜，鄢文豪，白旭峰，邢永忠. 多效性基因Ghd7调控水稻剑叶面积[J]. 遗传, 2012, 34(7): 901-906.

TAN Cong, WENG Xiao-Yu, YAN Wen-Hao, BAI Xu-Feng, XING Yong-Zhong. Ghd7, a pleiotropic gene controlling flag leaf area in rice[J]. HEREDITAS, 2012, 34(7): 901-906.

参考文献

[1] Gladun IV, Karpov EA. Distribution of assimilates from the flag leaf of rice during the reproductive period of development. Russ J Plant Physiol, 1993, 40(1): 215-219.
[2] Yonezawa K. Yield components. In: Science of the Rice Plant vol. III Genetics. Tokyo, Japan, 1997: 400-412.
[3] Yue B, Xue WY, Luo LJ, Xing YZ. QTL analysis for flag leaf characteristics and their relationships with yield and yield traits in rice. Acta Genet Sin, 2006, 33(9): 824-832.
[4] Yan JP, Zhu J, He CX, Benmoussa M, Wu P. Molecular marker-assisted dissection of genotype× environment interaction for plant type traits in rice (Oryza sa-tiva L.). Crop Sci, 1999, 39(2): 538-544.
[5] Kobayashi S, Fukuta Y, Morita S, Sato T, Osaki M, Khush GS. Quantitative trait loci affecting flag leaf development in rice (Oryza sativa L.). Breed Sci, 2003, 53(3): 255-262.
[6] Mei HW, Li ZK, Shu QY, Guo LB, Wang YP, Yu XQ, Ying CS, Luo LJ. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross populations. Theor Appl Genet, 2005, 110(4):649-659.
[7] Yoon DB, Kang KH, Kim HJ, Ju HG, Kwon SJ, Suh JP, Jeong OY, Ahn SN. Mapping quantitative trait loci for yield components and morphological traits in an advanced backcross population between Oryza grandiglumis and the O.sativa japonica cultivar Hwaseongbyeo. Theor Appl Genet, 2006, 112(6): 1052-1062.
[8] Zhang Y, Wang J, Xu C, Xing Y. Molecular dissection of genetic basis of significant correlation among five morphological traits in rice. Chin Sci Bull, 2010, 55(27): 3154-3160.
[9] Thomson MJ, Tai TH, McClung AM, Lai XH, Hinga ME, Lobos KB, Xu Y, Martinez CP, McCouch SR. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107(3): 479-493.
[10] Jiang S, Zhang X, Wang J, Chen W, Xu Z. Fine mapping of the quantitative trait locus qFLL9 controlling flag leaf length in rice. Euphytica, 2010, 176(3): 341-347.
[11] Wang, P, Zhou GL, Yu HH, Yu SB. Fine mapping a major QTL for flag leaf size and yield-related traits in rice. Theor Appl Genet, 2011, 123(8): 1319-1330.
[12] Price AH. Believe it or not, QTLs are accurate! Trends Plant Sci, 2006, 11(5): 213-216.
[13] Fan C, Xing Y, Mao H, Lu T, Han B, Xu C, Li X, Zhang Q. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet, 2006, 112(6): 1164-1171.
[14] Li Y, Fan C, Xing Y, Jiang Y, Luo L, Sun L, Shao D, Xu C, Li X, Xiao J, He Y, Zhang Q. Natural variation in GS5 plays an important role in regulating grain size and yield in rice. Nat Genet, 2011, 43(12): 1266-1269.
[15] Song XJ, Huang W, Shi M, Zhu MZ, Lin HX. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat Genet, 2007, 39(5): 623-630.
[16] Weng J, Gu S, Wan X, Gao H, Guo T, Su N, Lei C, Zhang X, Cheng Z, Guo X, Wang J, Jiang L, Zhai H, Wan J. Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight. Cell Res, 2008, 18(12): 1199-1209.
[17] Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, Angeles ER, Qian Q, Kitano H, Matsuoka M. Cytokinin oxidase regulates rice grain production. Science, 2005, 309(5735): 741-745.
[18] Xue W, Xing Y, Weng X, Zhao Y, Tang W, Wang L, Zhou H, Yu S, Xu C, Li X, Zhang Q. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40(6): 761-767.
[19] Yan WH, Wang P, Chen HX, Zhou HJ, Li QP, Wang CR, Ding ZH,

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多效性基因Ghd7调控水稻剑叶面积

Ghd7, a pleiotropic gene controlling flag leaf area in rice

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