[1] Schmidt MA, Parrott WA. Quantitative detection of trans-genes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) by real-time polymerase chain reaction. Plant Cell Rep, 2001, 20(5): 422-428.[2] 陈颖, 徐宝梁, 苏宁, 葛毅强, 王曙光. 实时荧光定量PCR技术在转基因玉米检测中的应用研究. 作物学报, 2004, 30(6): 602-607.[3] Beltrán J, Jaimes H, Echeverry M, Ladino Y, López D, Duque MC, Chavarriaga P, Tohme J. Quantitative analysis of transgenes in cassava plants using real-time PCR tech-nology. In Vitro Cell Dev Biol-Plant, 2009, 45(1): 48-56.[4] 敖金霞, 高学军, 仇有文, 郭士成. 实时荧光定量PCR技术在转基因检测中的应用. 东北农业大学学报, 2009, 40(6): 141-144.[5] Guillemaut P, Maréchal-Drouard L. Isolation of plant DNA: A fast, inexpensive, and reliable method. Plant Mol Biol Rep, 1992, 10(1): 60-65.[6] 方宣钧, 孔巍, 金芜军. 快速一步法(ROSE法)提取DNA应用于RAPD-PCR扩增. 高技术通讯, 1997, 7(10): 40-43.[7] 陈绍宁, 崔继哲. 快速提取番茄总DNA应用于规模化PCR检测. 北方园艺, 2004, (3): 46-47.[8] 刘小花, 张岚岚, 朱长青, 陈昆松, 徐昌杰. Micro-Tom番茄矮化微型机制及其在植物功能基因组学研究中的应用. 遗传, 2008, 30(10): 1257-1264.[9] Higgins CM, Hall RM, Campbell PR, Dietzgen RG. PCR rescue and analysis of transgene sequences directly from crude extracts of transgenic embryos and plants. Plant Mol Biol Rep, 2000, 18(3): 285a-285g.[10] Bruno AK, Wetzel CM. The early light-inducible protein (ELIP) gene is expressed during the chloro-plast-to-chromoplast transition in ripening tomato fruit. J Exp Bot, 2004, 55(408): 2541-2548.[11] Ruijter JM, Ramakers C, Hoogaars WMH, Karlen Y, Bakker O, van den Hoff MJB, Moorman AFM. Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res, 2009, 37(6): e45.[12] 王延华, 董坚, 齐建国. 基因克隆理论与技术(第二版). 北京: 科学出版社, 2009: 145-148.[13] Mogilner N, Zutra D, Gafny R, Bar-Joseph M. The persistence of engineered Agrobacterium tumefaciens in agroinfected plants. Mol Plant-Microbe Interact, 1993, 6(5): 673-675.[14] Tang H, Ren Z, Krczal G. An evaluation of antibiotics for elimination of Agrobacterium tumefaciens from walnut somatic embryos and for the effects on the proliferation of somatic embryos and regeneration of transgenic plants. Plant Cell Rep, 2000, 19(9): 881-887.[15] Nain V, Jaiswal R, Dalal M, Ramesh B, Kumar PA. Polymerase chain reaction analysis of transgenic plants contaminated by Agrobacterium. Plant Mol Biol Rep, 2005, 23(1): 59-65.[16] Li ZW, Hansen JL, Liu Y, Zemetra RS, Berger PH. Using real-time PCR to determine transgene copy number in wheat. Plant Mol Biol Rep, 2004, 22(2): 179-188.[17] Yi CX, Zhang J, Chan KM, Liu XK, Hong Y. Quantitative real-time PCR assay to detect transgene copy number in cotton (Gossypium hirsutum). Anal Bio-chem, 2008, 375(1): 150-152.[18] Bubner B, Baldwin IT. Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Rep, 2004, 23(5): 263-271.[19] Bubner B, Gase K, Baldwin IT. Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR. BMC Biotechnol, 2004, 4: 14. |